摘要
目的 探讨肝缺血再灌注损伤的作用机制。方法 采用大鼠部分肝缺血再灌注模型 ,将健康雄性SD大鼠 2 4只随机分为三组 :A组 (手术对照 ) ,B组 (肝缺血 90min) ,C组 (肝缺血 90min再灌注 1 2 0min)。观察每一动物肝组织病理切片 ;分别检测血浆谷草转氨酶 (AST)、谷丙转氨酶(ALT)、乳酸脱氢酶 (LDH)、肿瘤坏死因子 (TNF α)、白介素 1 β(IL 1 β)浓度 ;测定肝组织中髓过氧化物酶 (MPO)含量。结果 肝缺血再灌注后 ,光镜下大鼠肝组织有明显的肝血窦和中央静脉瘀血 ,内皮细胞及肝细胞普遍水肿变性 ;C组肝细胞坏死较B组明显 ;血浆中肝功能酶学指标显著升高 ,(B、C组与A组比及C组比B组P均 <0 0 1 ) ;肝组织中MPO活性升高 ,以再灌注 1 2 0min组为著 (C组比A组P <0 0 1 ) ;与血浆中TNF α、IL 1 β的变化趋势相同 (TNF α :C组比A组P <0 0 5 ,IL 1 β:B、C组比A组P均 <0 0 1 )。结论 肝脏微循环障碍是肝缺血再灌注损伤的病理基础 ;TNF α、IL 1
Objective To investigate hepatic I/R injury and the role of neutrophils in ischemia reperfused rat liver. Methods 24 healthy male SD rats were randomly divided into there groups;group A was served as sham operated control group, group B was subjected to 90min lobar, rather than totlal hepatic ischemia and group C was subjected to 120 min reperfusion after 90 min ischemia. In addition to histologic sections of liver in each group, plasma tumor necrosis factor α(TNF α),interleukin 1β(IL 1β),alanine aminotransferase(ALT), aspartate aminotransferase (AST), lactate dehydrogenase(LDH) levels and neutrophils in infiltration in liver tissue(content of myeloperoxidase) were respectively examined in A, B and C group. Results There was histologic evidence of liver injury. Plasma ALT, AST, LDH ,as evidenced by significant increase of developed after 120 min reperfusion. In group C , plasma TNF α?IL 1βlevels, as compared with A group, revealed a important elevation .The content of myeloperoxidase(MPO) in liver, a index of neutrophilic infitration in liver tissue, went up to 2 518±0 901(U/g) from 0 108±0 005(U/g) in control group, at the end of 120 min reperfusion, following 90 min of ischemia. Conclusion Proinformation cytokines such as TNF α?IL 1βmediate neutrophil induced hepatic ischemia/reperfusion injury.
出处
《中华肝胆外科杂志》
CAS
CSCD
2002年第6期366-368,共3页
Chinese Journal of Hepatobiliary Surgery
