摘要
目的 研究肥大细胞 (MC)在幽门螺杆菌 (Hpylori)发病中的作用。方法 选胃镜检查者 87例 ,其中诊断十二指肠溃疡 (DU) 3 3例 ,浅表性胃炎 45例 ,糜烂性胃炎 9例。取胃窦、体部活检分别作组织病理学 (HE染色 )及Hpylori检查 (快速尿素酶、培养及Warthin Starry银染 ) ,免疫组织化学染色 (ABC法 )后分别计算粘膜及粘膜下的完整和脱颗粒MC数。结果 MC计数在Hpylori阳性和Hpylori阴性组间无明显差别 (P >0 .0 5 ) ,但前者粘膜内MC脱颗粒者明显多于后者 (分别为 43 %和 2 3 % ,P <0 .0 1)。MC计数与粘膜炎症程度无明显关系 ,但在活动性炎症时显著高于非活动性炎症组 (P <0 .0 5 )。各病变组间MC计数也无显著差异。
Aim To investigate the effect of gastric mast cell and its degranulation in the pathogenesis of H.pylori associated mucosal inflammation.Methods An avidin biotin complex immunohistochemical procedure was used to determine mast cell character and its distribution in formaldehyde fixed gastric mucosa from the patients with duodenal ulcer ( n =33),superficial gastritis ( n =45) and erosive gastritis( n =9).The mucosa was also examined for histopathology by hematoxylin eosin staining and H.pylori status by rapid urease test, culture and Warthin Starry silver staining.Degranulated and undegranulated mast cells in mucosa and submucosa were counted respectively.Results 78.8% of the DU patients and 53.3% of the patients with superfacial gastritis and 77.8% of the patients with erosive gastritis were H.pylori positive.The number of mast cells was not increased in the gastric mucosa with H.pylori infection,but the number of degranulated mast cells were significantly increased (23% vs 43%, P <0.05),which was closely associated with inflammatory activity in the gastric mucosa.No certain correlation was revealed between mast cell count and inflammation grade of the mucosa, but a significant difference was observed between the patients with active inflammation and those with non active inflammation (124/mm 2 vs 82/mm 2).The numbers of degranulated mast cell were also significantly increased in the patients with erosive gastritis and duodenal ulcer (18% and 23%).Conclusions It suggests that mast cell degranulation plays an important role in the development of gastric inflammation when H.pylori infection happened and that mast cell causes mucosal injury by releasing histamine and other proinflammatory mediators.
出处
《胃肠病学和肝病学杂志》
CAS
2002年第2期124-126,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
幽门螺杆菌
肥大细胞
胃粘膜
Helicobacter pylori Mast cell Gastric mucosa
