摘要
作者用ABC法和D-PAP法检测了59例慢性乙型肝炎患者肝内HBcAg及血清HBV-DNA、抗-HBe。结果,肝内HBcAg的检出和含量与肝损伤呈负相关趋势,提示CAH肝损伤程度不直接与HBV复制有关,且表明血清HBV-DNA能很好地反映肝内HBcAg存在情况,在血清中已出现抗-HBe的病人中可检出肝内HBcAg(39％)及血清HBV-DNA(45％),所以不能认为抗-HBe是HBV复制停止及传染性减弱的指标。
We detected the presence and distribu-tion of HBcAg in the liver by immunohisto-chemistry (ABC method) and the presenceof HBV-DNA in serum (spot hybridization)and anti-HBe in serum (ELISA) from 59cases of hepatitis B hospitalized in our hos-pital,including 47 cases of CAH, 5 casesof CPH, and 7 cases of subacute fulminanthepatitis.1. HBchg in the liver was detected in 25 out of 47 cases (53％) of CAH, in 2 outof 5 cases of CPH and in 4 out of 7 casesof subacute fulmital hepatitis. The totalpercentage was 53％(31/59). 2. There was no positive correlationbetween HBV replication activity and liverdisease activity (P>0.05). Our results didnot support the hypothesis that suggests adirect cytopathic effect of HBV. Oppositely,the fact was that the presence, the amountand the patterns of HBcAg in the liver, andthe pressence of HBV-DNA in serum werepredominant in mild CAH compared withthose in severe CAH, predominant inCAH without cirrhosis compared withthose in CAH with cirrhosis. Therewas a tendency of inverse correlationbetween HBV replication activity and liverdisease activity.The results above were inline with the concept that HBcAg expressedon the surface of infected hepatocytesmay be relevant target for T lymphocytecytotoxicity.The results have suggested thatan immune response to HBV is present,leading to the destruction of most infectedcells. 3. There was a positive correlation be-tween HBV-DNA in serum and HBcAg inthe liver (P<0.005), indicating that HBV-DNA in serum can represent HBV replication. 4. Among anti-HBe positive patients, 39％(11/28) showed the presence of HBcAg inthe liver and 45％(10/22) HBV-DNA in se-rum, indicating that HBV replication maycontinue even after the seroconversion fromHBcAg to anti-HBe.
出处
《华西医科大学学报》
CAS
CSCD
1989年第2期203-207,共5页
Journal of West China University of Medical Sciences
关键词
乙型肝炎
抗-HBE
血清学
乙型肝炎
Mechanisms of chronic hepatitis B
HBVM
HBcAg
HBV-DNA
Anti-HBe
ABC method
