摘要
目的 观察兔自体骨髓间充质干细胞 (MSCs)体内复合移植的成骨能力 ,以寻求理想的MSCs载体。方法 将 10只新西兰大白兔随机分成A、B两组 ,从自体骨髓中分离出MSCs,体外培养并扩增后分别与相同大小的小牛脱钙骨 (DBCB)、自固化磷酸钙人工骨 (CPC)复合移植于两组大白兔左侧骶棘肌中 ,同时右侧骶棘肌分别植入相同大小的DBCB、CPC作空白对照 ;16周后取出标本 ,观察成骨情况并行组织学检查。结果 A组 5例 :MSCs+DBCB侧均发现有新骨组织形成 ,单纯DBCB侧3例被完全吸收、降解 ,2例大部分吸收、降解 ;B组 5例 :MSCs +CPC侧和单纯CPC侧均未见骨组织形成 ,植入物也无明显吸收。结论 自体骨髓MSCs在适合载体负载下可在体内自动分化成骨 ,DBCB是MSCs的良好载体之一。
Objective To observe osteogenicity of in vivo composite grafting of autologous marrow mesenchymal stem cells (MSCs) of rabbits and search for ideal matrix of MSCs. Methods The autologous MSCs of rabbits were isolated from bone marrow, grown in culture, and loaded onto demineralized bovine cancellous bone (DBCB) and calcium phosphate cement (CPC) respectively and then implanted separately into the left sacrospinal muscle of two groups of rabbits (group A with MSCs+DBCB and group B with MSCs+CPC). Meanwhile, the pure DBCB and CPC were separately implanted into the right sacrospinal muscle of two groups of rabbits (group A with pure DBCB and group B with pure CPC) as the blank control. 16 weeks later, the animals were killed and the specimens harvested to observe the formation of bone. The histological sections of specimens were stained and observed. Results In group A (of five rabbits),abundant new bone was found to have formed in all specimens of MSCs DBCB while the implanted pure DBCB was wholly or mostly absorbed and degraded.In group B (of five rabbits),no new bone was found in any specimens of MSCs CPC or pure CPC and none of the implanted specimens was markedly absorbed. Conclusion The autologous MSCs loaded onto appropriate matrix can form bone in vivo and DBCB is the satisfactory matrix for MCS.
出处
《临床骨科杂志》
2002年第2期81-85,共5页
Journal of Clinical Orthopaedics
基金
安徽省 2 0 0 2年度重点科研项目 (编号 :0 10 2 3 0 2 2 )
