摘要
目的 :分析染色体 9p2 1.3- 2 3区域等位基因杂合性缺失 (lossofheterozygosity ,LOH)情况 ,确定其在中国人喉鳞癌中发生频率和最小缺失区域 ,为定位克隆喉鳞癌相关抑癌基因提供线索。方法 :应用 9个微卫星多态性标记对 2 4例喉鳞癌组织及其对应的正常远端肺组织进行了同位素标记的聚合酶链反应和变性凝胶电泳 ,并探讨各位点LOH与病理分级和病理分期的关系。结果 :2 4例喉鳞癌组织中 ,共有 2 3例 (95 .83% )存在至少一个位点的LOH ,丢失频率最高的位点位于9p2 2 - 2 3的D9S1782 (10 0 % )其次是位于 9p2 2 - 2 3的D9S16 2 (85 .71% )。结论 :在 9p2 1.3- 2 3区域可能存在两个或两个以上的与喉鳞癌发生相关的候选抑癌基因 ,分别位于 9p2 2 - 2 3的D9S1782和D9S16
Objective: To determine the frequency and common deletion region of allelic losses on chromosome 9p21.3-23 in the laryngeal squamous cell carcinoma and facilitate the isolation of the candidate tumor suppressor genes associated with the development of laryngeal squamous cell carcinoma. Methods: Using 9 microsatellite markers, we mapped the region deleted frequently on 9p21.3-23 of tumor tissues of primary laryngeal squamous cell carcinoma surgically resected in 24 cases. We also analyzed the relationship between the loss of heterozygosity (LOH) on each locus and the pathological grade and stage. Results: Among 24 cases, 23 (95.83%) samples showed allelic loss of the 9p21.3-23 locus in at least one. The most frequent LOH loci were located in two regions: D9S1782 with in 7 of 7(100%) and D9S261 with in 6 of 7 (85.71%) locus on 9p21.3-23. Conclusion: These data have shown an interval of common deletion, encompassing D9S1827, D9S261 which might localize at least two candidate tumor suppressor genes in 9p21-23 region, which associated with occurence of laryngeal squamous cell carcinoma.
出处
《大连医科大学学报》
CAS
2002年第2期81-83,共3页
Journal of Dalian Medical University
基金
国家重点基础研究发展规划项目 (G19980 5 12 0 7)
关键词
喉肿瘤
鳞状细胞癌
杂合性缺失
抑癌基因
定位克隆
squamous cell carcinoma
laryngeal neoplasms
tumor suppressor gene
LOH
position clone.
