摘要
本文研究了半胱氨酸(Cys)及其结构类似物半胱胺(MEA),N-乙酰半胱氨酸(NAC)、胱胺(CSSC),γ-氨丙基甲基异硫脲(APMT),对离体大鼠Langendortff心脏缺血再灌所致心律失常的保护作用.给药(0.1,0.6,3,6μmol/min)10min,结扎LAD 10 min再灌5 min。结果表明含游离巯基的Cys,NAC,MEA在0.6和3.6 μmol/min时,与生理盐水对照组相比可显著降低室颤发生率(P<0.01～0.001),缩短室颤时程(P<0.01～0.001).CSSC和APMT未见明显保护作用。此外,Cys,NAC和MEA还可明显增加冠脉流量(P<0.01),CSSC和APMT则反而使冠脉流量降低。
Protective effects of cysteine (Cys), N-acetylcysteine(NAC), cysteamine(MEA), cystamine (CSSC) and aminopropylmethylisothiourea(APMT) on ischemia/reperfusion induced arrhythmias were studied in isolated Langendorff perfused rat hearts. The arrhythmias were caused by ligation of the anterior descending branch of the left coronary artery for 10min and reperfused for 5 min. The drugs were dissolved in saline (NS) and perfused through a peristaltic pump system at 0.1, 0.6 or 3.6 μmol/min(n=10), starting from 10min before ligation up to 5 min after reperfusion. The control hearts were perfused with NS. The results showed that Cys, NAC and MEA persused at 0.6～3.6μmol/min significantly reduced the incidence of ventricular fribrillation(VF), which were 80～90% in control and 0～20% in 3 treated groups, with P<0.01～0.001. The duration of ventricular tachycardia (VT)+VF was 3.0±1.6 min in control and were 0.2±0.2, 0.2±0.1 and 1.2±2.1 min in Cys, NAC and MEA groups, respectively (with P<0.01-0.001). Coronary flow (CF) were remarkably reduced to about 50% during ligation in NS, but remained at normal levels in three treated groups. There were no significant protective effects on arrhythmias in CSSC and APMT perfused hearts. CF of CSSC and APMT groups were even less than those of control. The structure-activity analysis suggested that the SH group may play a crucial role in the protective effect of SH compounds on ischemia/reperfusion induced arrhythmias. The mechanism of protection was briefly discussed in this paper.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第2期91-95,共5页
Acta Pharmaceutica Sinica
关键词
巯基化合物
心肌缺血
保护作用
Cysteine
Cysteamine
Cystamine
N-acetylcysteine
Myocardial ischemia/reperfusion.
