摘要
以李属植物 9个种的 2 0个材料为研究对象 ,用RAPD技术对其进行亲缘关系分析。在建立适合李属植物的PCR RAPD反应体系的基础上 ,从 45个随机引物 ( 1 0mer)中筛选出 2 4个 ,对所有供试材料进行扩增 ,共获得 2 4张DNA指纹图谱 ,3 2 6条DNA谱带 ,其中有 3 1 1条为多态带。建立了基于RAPD的李属植物亲缘关系树形图 ,树形图的聚类结果与经典的李属植物的起源、分布和分类基本一致。另外 ,根据聚类结果 ,作者认为 :1 )乌苏里李是中国李的一个变种而非一独立的种 ;2 )杏李是一李杏杂种且与杏有较近的亲缘关系 ;3 )从分子水平证明了欧洲李 (PrunusdomesticaL.)是由樱桃李 (P .cerasiferaEhrh.)和黑刺李 (P .spinosaL .)自然杂交形成的后代。
In this study, nine species of Prunus (20 materials)were analyzed using random amplified polymorphic DNA markers. 24 primers were screened from 45 arbitrary 10 mer primers, and a total of 326 DNA bands were amplified, 311 of which (95.4%) were polymorphism. According to the data,a tree diagram of phylogenetic relationship was constructed using UPGMA in NYSYS. The results showed that: 1) P. ussuriensis was a variety of P. salician , not a independent species; 2) P. simonii was a hybrid of P. salician and P. armaniaca and was closely related to P. armaniaca ; 3) P. domestica was a offspring formed by naturally crossing between P. cerasifera and P. spinosa .
出处
《园艺学报》
CAS
CSCD
北大核心
2002年第3期218-223,共6页
Acta Horticulturae Sinica
