摘要
用免疫过氧化物酶技术对44例肝内胆管癌作单克隆与多克隆角蛋白抗体免疫组化观察,发现角蛋白在癌细胞内分布与癌组织分化程度有关。高分化乳头状腺癌以膜型为主,癌细胞顶端胞浆淡染;管状腺癌则多集中于癌细胞腔面顶端,在腔缘形成连续的深染带;低分化腺癌阳性部位则以浆型及核周浆型为主;细胆管上皮癌呈膜型分布。
Forty-four cases of intrahepatic cholangiocarcinoma of various degrees of differentiation were studied by immunoperoxidase using monoclonal and polyclonal antibodies.Localization of keratin in the cancer cells was related with its differentiation.In well-differentiated papillary adenocarcinoma, keratin was of membranous type, with apical cytoplasm faint-stained.In glandular form adenocarcinoma, keratin was located at the apex of the cancer cells forming a deep-stained consecutive band. In poorly differentiated tumor, keratin presented as cytoplasmic type or in perinuclear distribution keratin in cholangiocellular carcinoma was predominantly membranous.
出处
《湖南医科大学学报》
CSCD
1989年第3期249-252,共4页
Bulletin of Hunan Medical University
关键词
肝内胆管癌
角蛋白
免疫组化
liver neoplasms
cholangiocarcinoma
keratin
immunoenzyme technics
histocytochemistry
