摘要
采用YEPD培养基诱导发酵生产重组抗菌肽。经 72h培养 ,由重组酵母表达并分泌到培养基中的抗菌肽活性可高达 5 0 0 0U/ml。用纸片扩散法和试管稀释法测定重组抗菌肽对水产养殖常见病原菌的抑菌效果。结果表明 ,抗菌肽对水产养殖常见病原菌嗜水气单胞菌 (Aeromonashydrophila)、梅氏弧菌 (Vibriometschnikovi)、迟钝爱德华氏菌 (Edwardsiellatarda)及温和气单胞菌 (A .sobria)有较好的抑菌效果 ,最小抑菌质量浓度为 4 μg/ml、杀菌质量浓度为 8μg/ml。对抗菌肽抑菌过程的超微结构观察表明 ,抗菌肽的抑菌方式是通过作用于细菌的细胞膜而导致菌体裂解死亡。本研究目的在于探讨基因工程抗菌肽在水产养殖中的应用。
The recombinant antimicrobial peptides was prepared by ferment in YEPD (30 ℃,pH 6.0～6.5) for 36 h and induced by methanol. After 72 h ferment,the activity of recombinant antimicrobial peptides expressed by recombinent yeast in the medium can reach as high as 5 000 U/ml. By paper diffusion method and tube dilution method,the antibacterial effects of antimicrobial peptides on the fish pathogenic bacteria Aeromonas hydrophila,Vibrio metschnikovi,Edwardsiella tarda and Aeromonas sobria are determined that the minimal inhibitory concentration (MIC) is 4 μg/ml and the minimal lethal dose ( MLD) is 8 μg/ml. The ultrastructure observation under electron microscopy shows that the bactericidal machinism of antimicrobial peptides is to make the bacterial cell lytic, which leads to the becteria death.
出处
《中国水产科学》
CAS
CSCD
北大核心
2002年第2期152-156,共5页
Journal of Fishery Sciences of China
基金
农业部"九五"重点渔业科技项目 (95 -B -96-0 2 -0 8-0 2 )
关键词
制备
病原菌
重组抗菌肽
抑菌效果
嗜水气单胞菌
梅芪弧菌
迟纯爱德华氏菌
温和气单胞菌
gene recombinational antimicrobial peptides
antibacterial effect
Aeromonas hydrophila
Vibrio metschnikovi
Edwardsiella tarda
Aeromonas sobria
