摘要
目的 :优化金银花中绿原酸的提取工艺。方法 :将金银花乙醇回流前 ,用纤维素酶和果胶酶分别或联合处理 ,探讨酶的用量、处理时间、处理温度及酶的联合作用对金银花提取物得率和绿原酸得率的影响。结果 :纤维素酶处理能显著提高金银花提取物得率和绿原酸得率 (8.15 g/ 10 0g) ,酶处理最适温度为 4 0℃～ 5 0℃。纤维素酶和果胶酶的联合处理对绿原酸得率影响不明显 ,但能显著提高提取物得率。采用该工艺绿原酸得率最高可达到 8.32 %。结论
Objective: To optimize the extraction process of chlorogenic acid in flos lonicerae.Methods: The flos lonicerae was treated by means of cellulase and/or pectolase before extracted by ethanol reflux, studying the enzyme dosage, treatment time, treatment temperature how to influence the percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction.Results: The percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction could be obviously increased by the cellulase treatment, 8.15g chlorogenic acid could be extracted from 100g flos lonicerae. The optimum temperature of treatment was 40℃～50℃; The percentage of extraction was higher by the combined treatment of cellulase and pectilase than by cellulase only, but the amount of chlogenic acid was not obviously different between these two methods.Conclusion: The amount of chlorogenic acid in extrction could be increased by enzymic treatment.
出处
《中成药》
CAS
CSCD
北大核心
2002年第6期416-418,共3页
Chinese Traditional Patent Medicine
关键词
金银花
绿原酸
酶处理
提取工艺
Flos lonicerae
Chlorogenic acid
enzymic treatment
extraction process
