摘要
目的 :探讨缺氧预适应小鼠中一氧化碳对缺氧诱导因子 - 1表达和缺氧耐受时间的影响。方法 :小鼠腹腔注射生理盐水 (NS)或氯化高铁血红素 (hemin)后 ,进行重复缺氧 4次实验 ,Western印迹法检测海马中缺氧诱导因子 - 1α(HIF - 1α)的表达 ,并记录缺氧耐受时间。结果 :缺氧 1次和 2次 ,注射NS和注射hemin小鼠海马中HIF -1α的水平基本相同 ,缺氧耐受时间相似。重复缺氧 3次和 4次 ,注射hemin小鼠海马中HIF - 1α含量少于注射NS小鼠 ,缺氧耐受时间低于注射NS小鼠 (P <0 0 5 )。结论 :在缺氧预适应模型中 ,一氧化碳能减少海马中HIF - 1α的表达 ,进而降低缺氧耐受时间。
AIM: To study the effect of carbon monoxide (CO) on hypoxia inducible factor-1α (HIF-1α) expression and on hypoxic tolerance duration in hypoxia preconditioned mice. METHODS: Mice were injected with normal saline (NS) or hemin intraperitoneally. Western blot was used to detect HIF-1α in hippocampus. The tolerant duration of each hypoxic run was recorded. RESULTS: After 1 and 2 runs of hypoxia exposure, there were no significant differences in HIF-1α expression and hypoxic tolerance duration between N and hemin injection groups. After 3 and 4 runs of hypoxia exposure, the content of HIF-1α was lower and hypoxic tolerance duration was shorter ( P <0.05) in hemin injection groups than that in NS injection groups. CONCLUSION: CO could decrease the expression of HIF-1α, and decrease hypoxic tolerance duration in hypoxia preconditioned mice. The mechanism may be that CO binds to hemoprotein and inhibits hemoprotein oxygen sensor.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第5期494-496,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (396 70 2 71)
北京市自然科学基金资助项目 (796 2 0 0 9)
