摘要
了解雌激素受体α(estrogen receptor alpha,ERα)在大鼠脑的分布及大鼠下丘脑视前区雌激素受体样阳性神经元的生后发育规律。用免疫组织化学反应方法结合图像分析仪检测雌性大鼠下丘脑视前区雌激素受体样阳性神经元的数量和灰度值。 ERα分布于 Calleja岛、梨形核、外侧隔核、基底前脑胆碱能神经元各群、终纹床核、下丘脑内侧视前区、室周核、腹内侧核、弓状核和结节乳头核、再连合和前内侧丘脑核、杏仁核复合体、梨形皮质和穹窿下器官。相比之下 ,皮质和海马内仅见几个分散的 ERα样阳性神经元。而纹状体内未见 ERα样阳性神经元。ERα免疫反应产物主要位于细胞核内 ,蓝黑色。在成年雌性大鼠下丘脑内侧视前区 (m edial preoptic area,MPA)神经元的胞浆和突起内可见较弱的 ERα免疫反应产物。在MPA内 ,生后 1天可见 ERα表达 ,随着大鼠的生后发育 ,成年时达到高峰。与成年大鼠比较 ,老年雌性大鼠雌激素受体样阳性神经元数量减少 10 .0 5 % ,P>0 .0 5 ,差异无显著性 ;平均灰度减少 41.5 7% ,P<0 .0 5 ,差异有显著性。老年雌性大鼠下丘脑 MPA内 ERα表达下调 ,可能与卵巢功能减退而导致情感。
To investigate the distribution of estrogen receptor α (ERα) in the rat brain and profiles of ER α immunoreactive (ER α ir) neurons in the preoptic area of hypothalamus during postnatal development Immunohistochemistry (ABC) with an antibody directed against human full length ER α was employed ER α expression and ER α ir neurons were analysed with an image analyser by counting and measuring the grey degrees ER α ir positive neurons were distributed within the islands of Calleja, endopiriform nucleus, lateral septum, subfields of the basal forebrain, bed nucleus of the stria terminalis, medial preoptic region, periventricular, ventromedial, arcuate and tuberal mammillary nuclei of the hypothalamus, reunions and anterior medial thalamic nuclei, amygdaloid complex, piriform cortex and subfornical organ In contrast, only a few scattered ER α labeled neurons were found in the cortex and hippocampus, while no ER α ir neurons were found in the striatum ER α staining consisted of blue/black reaction products which is mainly localized in the nucleus The weak staining of ER α in the cytoplasm and processes was found in MPA of the hypothalamus in adult female rats, where ER α immunostaining was present from the 1st postnatal day Most densely stained ER α neuronal bodies and processes were present in the adult, and quantitative data showed that ER α reached its peak in the adulthood In old age the number of ER α ir positive neurons was decreased by 10 05%,which was no significant difference ( P >0 05) However, the mean grey degree was decreased by 41 57%, showing significant difference ( P <0 05) The down regulation of ER α in the brain might be associated with the declining ovary function and linked to the changes in emotion and loss of memory during aging.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2001年第4期383-386,468,共5页
Chinese Journal of Histochemistry and Cytochemistry
