摘要
利用RAPD技术 ,从 2 48个随机寡聚核苷酸 (10bp)中筛选出 13个引物能在供试的三组三系杂交水稻及亲本间扩增出 43条稳定性较好的多态性片段 ,其中 6个引物能在供试材料间扩增出 2 0个强的多态性标记。利用这些标记能有效地区分各组合中不育系、保持系、恢复系和F1,并能看出各组合中不育系与保持系、不育系与恢复系。
A total of 248 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) techniques with the genome DNA of three groups of three-line hybrid rice and their parents.Thirteen primers produced 43 polymorphism fragments.Six primers of them produced 20 obviously repeatable polymorphic markers among rice lines tested.Using this RAPD markers,the hybrid rice combinations (sterile-line,maintainer-line,restorer-line and F 1)can be effectively identified,and the genetic relationship among them can be shown.
出处
《遗传》
CAS
CSCD
北大核心
2002年第3期283-287,共5页
Hereditas（Beijing)
基金
国家自然科学基金 (批准号 :39770 45 5 )
