摘要
以不同梨品种为材料,分别采用改良CTAB法、改良SDS法、SDS—CTAB和高盐低pH值法对梨基因组DNA进行了提取。结果表明:采用改良CTAB法在提取各种梨基因组DNA过程中表现最好且纯度最高;SDS法提取的基因组DNA产率最高,但纯度较低;高盐低pH值法产率和纯度均最低。
Different varieties of pears were used m extract genomic DNA of pears by using improved CTAB , improved SDS ,SDS-CTAB, high salt--low pH in this experiment. The results showed that improved CTAB method was superior to other methods and the purity reached to the highest; the yield reached to the highest by using SDS method, but the purity was lower; the.yield and purity was the lowest by using high salt-low pH method.
出处
《北方园艺》
CAS
北大核心
2007年第4期203-205,共3页
Northern Horticulture
关键词
梨
DNA
提取方法
Pear
DNA
Extraction methods
