摘要
本文用中药小檗碱作为探针分子,在0.01mol·L-1醋酸-醋酸钠缓冲体系中,用荧光和紫外-可见吸收光谱研究了贵金属离子Ag?、Au?、Pt?与DNA的相互作用。在三种贵金属离子中,Au?、Pt?离子对小檗碱-DNA体系均具有明显的荧光猝灭作用,而Ag?离子对该体系有强烈的荧光敏化作用。分别求出了三种贵金属离子与DNA的结合常数。三种贵金属离子与DNA结合能力的强弱顺序依次为:Au?>Ag?>Pt?。探讨了三种贵金属离子与DNA的作用机理及导致结合力不同的原因。
The interactions of Ag ??Au ??Pt ?ion with DNA were studied by fluoresc ence spectroscopy and absorption spectroscopy using berberine(BER)as the probe molecule.Obvious spectroscopic changes of the BER-DNA sys-tem in 0.01mol·L -1 HAC-NaAC buffer solution were observed after adding Ag ??Au??Pt ?ion to the binary system,respectively.The appraren t binding constants of these three me tal ions with DNA in the BER-DNA binary system were respectively obtained.According to the binding constants o btained,the order for the binding ab ilities of these three noble metal ions with DNA from the strongest to the weakest was as follows:Au?>Ag ?>Pt ?.Besides,the binding modes of three n oble metal ions to DNA as well as the possible causes of different binding abilities of three noble metal ions with DNA were also discussed in this paper.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2002年第7期665-670,共6页
Chinese Journal of Inorganic Chemistry
基金
南京大学985学科发展项目资助(No.2000)
