摘要
目的:探讨梗阻性胆管炎大鼠细胞免疫功能降低的发生机制及清热通下中药锦红片的影响.方法:♂SD大鼠24只建立急性梗阻性胆管炎模型,随机分为模型组(n=8)、锦红片治疗组(n=8)和单纯胆管梗阻组(n=8),检测血浆IL-2,CD_3^+,CD_4^+,CD_8^+,内毒素,胸腺指数,胸腺细胞凋亡指数及电镜下观察胸腺的超微结构及凋亡.结果:模型组IL-2,CD_3^+,CD_4^+和胸腺指数显著低于治疗组和单纯胆管梗阻组(IL-2:28.5±3.0 ng/L vs 33.9±3.6 ng/L,39.6±2.2 ng/L,P<0.05,P<0.01;CD_3^+:54.5%±5.5% vs 70.7%±4.8%,66.3%±7.1%,均P<0.01;CD_4^+:34.5%±8.3% vs 44.2%±3.3%,44.5%±4.2%,均P<0.01:胸腺指数:0.89±0.18 vs 1.10±0.13.1.12±0.24,均P<0.05),CD_8^+3组间没有统计学差异,血浆内毒素和凋亡指数明显高于治疗组和梗阻组(内毒素:0.85±0.14 Eu/mL vs 0.53±0.10 EU/mL,0.49±0.11 EU/mL,均P<0.01;凋亡指数:25.7±5.1 vs 15.8±5.5.9.0±3.1.P<0.05,P<0.01),模型组胸腺可见较多典型的凋亡细胞,结果显示经中药干预治疗后,免疫功能、内毒素血症和胸腺细胞凋亡有所改善,接近单纯胆管梗阻组水平.结论:梗阻性胆管炎大鼠存在免疫功能降低,胸腺细胞异常凋亡.锦红片对维持免疫机能的稳定有积极的意义.
AIM： To explore the mechanism of decreased cellular immune function and the effect of Jinhong Tablets, a Chinese herbal medicine, during the course of obstructive cholangitis.
METHODS： An obstructive cholangitis model was induced in 24 male Sprague-Dawley rats that were randomly divided into model （n = 8）, Jinhong Tablet-treated （n = 8） and simple obstructive cholangitis （n after modeling, all rats = 8） groups. Four days were sacrificed and the interleukin （IL）-2, CD3^＋, CD4^ and CDs^＋ levels in plasma were measured by enzyme liked immunosorbent assay （ELISA）. The endotoxin content was detected by a reagent kit. The index of apoptosis was determined by Tdt-mediated flu-orescein-dUTP nick end labeling （TUNEL）, and ultrastructure changes of the thymus and apoptosis were observed by electron microscopy.
RESULTS： Plasma IL-2, CD3^＋, and CD4^＋ levels and the thymus mass index in the untreated group were significantly lower than those in the Jinhong Tablet-treated and simple ligation groups （IL-2：28.5 ± 3.0 ng/L vs 33.9 ± 3.6 ng/L, 39.6 ± 2.2 ng/L, P 〈 0.05, P 〈 0.01; CD3^＋： 54.5% 5.5% vs 70.7% ± 4.8%, 66.3%± 7.1%, both P 〈 0.01; CD^4＋： 34.5% ＋ 8.3% vs 44.2% ± 3.3%, 44.5% ± 4.2%, both P 〈 0.01; thymus index： 0.89 ± 0.18 vs 1.10 ± 0.13, 1.12± 0.24, both P 〈 0.05）. There were no significant differences among the three groups with regard to the level of plasma CDs^＋, while plasma endotoxin and the thymus apoptosis index in the untreated group were significantly higher than in the Jinhong Tablet-treated and simple ligation groups （endotoxin： 0.85 ±0.14 EU/mL vs 0.53± 0.10 EU/mL, 0.49 ± 0.11 EU/ mL, both P 〈 0.01; thymus index： 25.7 ± 5.1 vs 15.8 ±5.5, 9.0 ± 3.1, P 〈 0.05, P 〈 0.01）. More typical apoptosis cells were seen in thymus tissue than in the untreated group. The results demonstrated that intervention by traditional Chinese medicine improved the immune function, endotoxemia and thymus cell apoptosis, even approaching the levels in the simple obstructive cholangitis.
CONCLUSION： Decreased cellular immune function and abnormal apoptosis of thymus cells exists in rats with obstructive cholangitis; Jinhong Tablets significantly improve the regulation and maintenance of immune function.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第18期2050-2053,共4页
World Chinese Journal of Digestology
基金
上海市重点学科课题资助项目
No T0304
上海市卫生系统百人计划课题经费资助项目
No.03HBR-29~~
关键词
中药锦红片
梗阻性胆管炎
胸腺
细胞免疫
凋亡
酶联免疫法
末端脱氧核苷酸转移酶缺口标记
linhong Tablet
Obstructive cholangitis
Thymus gland
Cellular immune
Apoptosis
Enzyme-linked immunosorbent assay
Tdt-mediated fluorescein-dUTP nick end labeling
