摘要
目的 :研究低分化滑膜肉瘤的临床病理学特点及其分子遗传学表现。方法 :收集低分化滑膜肉瘤标本 12例 ,采用形态学观察和免疫组化染色 ,并用RT PCR方法在石蜡包埋组织中检测SYT SSX融合mRNA表达。结果 :12例低分化滑膜肉瘤中小细胞型 4例 ,大细胞型 6例 ,高度恶性梭形细胞型 2例。 8例有随访资料 ,其中 4例死于肿瘤 ,平均生存时间 18个月。免疫组化表现为CK和 (或 )EMA阳性 ,以EMA阳性率较高 ,同时vimentin阳性。S 10 0蛋白也有较高阳性率 ,多呈局灶或散在阳性。RT PCR方法均可检测到SYT SSXmRNA表达 ,对照组 12例肿瘤包括恶性周围神经鞘膜瘤、尤因肉瘤和恶性血管外皮瘤SYT SSX检测全部阴性。结论 :低分化滑膜肉瘤有其形态学及免疫表型特点 ,分子遗传学检测SYT SSX融合mRNA有助于其诊断和鉴别诊断。
Purpose To investigate the clinicopathologic and molecular genetic characteristic of poorly differentiated synovial sarcoma. Methods Twelve cases of poorly differentiated synovial sarcoma were morphologically observed, immunohistochemically stained and detected for SYT SSX mRNA expression by reverse transcriptase polymerase chain reaction (RT PCR) in formalin fixed, paraffin embedded tissues. Results In 12 cases of poorly differentiated synovial sarcoma, 4 were small cell type, 6 were large cell type and 2 were high grade spindle cell type. Eight cases had follow up data, among them 4 died of disease with average follow up time of 18 months. Immunohistochemically, poorly differentiated synovial sarcomas were positive for cytokeratin and/or EMA with higher positive rate for EMA, at the same time all the cases were positive for vimentin. S 100 protein was positive in most cases with focally immunostaining. SYT SSX mRNA were expressed in all the cases of poorly differentiated synovial sarcoma as detected by RT PCR whereas control tumors including malignant peripheral nerve sheath tumor, Ewing's sarcoma and malignant hemangiopericytoma were all negative for SYT SSX. Conclusion Poorly differentiated synovial sarcoma has its morphologic and immunohistochemical characteristics and SYT SSX mRNA detection by molecular genetics can be useful in its diagnosis and differential diagnosis.
出处
《临床与实验病理学杂志》
CAS
CSCD
2002年第2期117-121,共5页
Chinese Journal of Clinical and Experimental Pathology
