摘要
目的 探讨阿仑膦酸钠 (alendronate ,ALN)对去势家兔骨质疏松的预防作用。方法 7月龄大白兔随机分成假手术组(shamoperation ,SHAM O)、去势对照组 (ovariectomy ,OVX)和实验组 (OVX +ALN)。SHAM O组行腹腔开关术 ,后两组行经腹腔双侧卵巢切除术 ,OVX +ALN组在切除卵巢后立即给予ALN 10mg·d-1喂服 ,饲养 12周。分别在术前和术后第 1,2 ,4,8,12周测定 3组的血清碱性磷酸酶 (alkalinephosphatase ,ALP)水平 ,尿钙肌苷比值 (calciumcreatinineratio ,Ca/Cr) ,用定量CT(quantitativecomputedtomography ,OCT)测定腰锥2～ 4 (lumbar2～ 4 vertebrate ,L2～ 4 )骨密度 (bonemineraldensity ,BMD)。第 12周末将全部家兔处死 ,取双侧股骨进行力学实验 ,取胫骨干骺端松质骨进行光镜切片观察。结果 与SHAM O组相比 ,OVX组血清ALP与尿Ca/Cr明显升高 ,OVX +ALN组血清ALP先轻度升高后 ,很快恢复到术前水平 ,尿Ca/Cr轻度下降。OVX组与另两组相比骨密度、股骨极限扭矩及 3点弯曲最大挠度显著下降 ,OVX +ALN组与SHAM O组间无明显差异。病理切片见OVX组骨小梁稀疏 ,变细 ,排列紊乱 ,另两组骨小梁粗细均匀 ,排列规整。结论 阿仑膦酸钠能有效预防去势后家兔的骨质疏松。
OBJECTIVE To investigate the preventive effect of alendronate (ALN) on osteoporosis induced by ovariectomy (OVX) in rabbits.METHODS The seven month old white rabbits were randomly divided into the sham operation (SHAM O) group,OVX group or OVX+ALN group.The later two groups were ovariectomized bilaterally.The OVX+ALN group was given ALN 10 mg·d -1 /animal orally after operation for 12 weeks.The serum alkaline phosphatase (ALP) and urinary calcium creatinine ratio (Ca/Cr) were measured before operation and 1,2,4,8 and 12 week postoperation respectively.The lumbar 2～4 vertebrate (L 2～4 ) bone mineral density (BMD) was measured 1 week prior to the operation and 12 week after the operation.At the end of 12 th week,all the rabbits were sacrificed.The femurs were used for mechanical measurement and the tibia for histomorphometrical examination.RESULTS The serum ALP and urinary Ca/Cr increased apparently in the OVX group while no significant difference was observed in the SHAM O group and the OVX+ALN group.L 2～4 BMD and mechanical parameters deteriorated significantly 12 weeks after operation,and light microscopy revealed a osteoporosis change in the OVX group.CONCLUSION Alendronate effectively prevented the osteoporosis induced by ovariectomy in rabbits.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2002年第6期427-429,共3页
Chinese Pharmaceutical Journal
