摘要
目的建立合适的同种异体肢体移植动物模式,研究X线照射供体对异体肢体移植的影响。方法Wistar大鼠为供者,SD大鼠为受者。按供者是否进行一次性X线照射(5 Gy)预处理将受者分为非照射组与照射组。切除SD大鼠的相应肢体,将Wistar大鼠的肢体移植给SD大鼠。术后长效青霉素抗炎治疗,观察宿主及移植物的变化情况。结果共进行大鼠异体肢体移植16例(非照射组与照射组各8例),非照射组成功6例,移植肢体存活时间为(12.0±2.4) d;照射组成功7例,移植肢体成活时间为(24.7±8.1) d,两组移植物的存活时间有显著差异(P<0.05)。结论X线照射供体可明显延长移植物的存活时间,对急性排斥反应有显著的防治作用。
Objective To establish appropriate animal models for observing the effects of X-ray irradiation on limb allograft rejection. Methods Wistar rats were used as donators and SD rats as recipients, the latter divided into 2 groups, namely irradiation group and non-irradiation group according to pretransplant treatment with or without X-ray radiation (5 Gy) on the part of the donators. The donor limbs were transplanted into SD rats who had their own limbs cut off, and the sciatic nerve, femoral nerve, and femoral artery and femoral vein were anastomosed in operation. After the operation, all the recipients were given benzathine benzylpenicillin, and their vital signs, together with changes of the allografts, observed. Results A total of 16 rats received transplantation that was successful in 6 rats of non-radiation group and 7 of irradiation group. The graft survival averaged 12.0±2.4 d in non-irradiation group and 24.7±8.1 d in irradiation group, showing significant difference in the lengths of survival between the 2 groups (P<0.05). Conclusion The graft survival time in rats can be significantly prolonged by pretransplant irradiation of the allograft, which also help control acute rejection of the allograft.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第6期509-511,共3页
Journal of First Military Medical University
基金
广东省重点科技攻关项目(2KM04901S)
