摘要
本实验采用反向Northern印迹杂交 (reverseNorthernblot)技术 ,对经反转录差异显示PCR(differentialdisplayre versetranscriptionalPCR ,DDRT PCR)获得的 5 6个差异片段 (cDNA)进行阳性片段的初步筛选 ,获得 8个候选阳性片段 ,经Northern印迹杂交实验 ,确证 5个阳性片段。实验表明该方法是一种快速检测和筛选阳性克隆片段的方法 ,并具有简便、经济的优点。它不仅可用于各种分离差异片段的方法中差异片段的初步筛选 。
Reverse Northern blot analysis against differential display reverse transcriptional PCR (DDRT PCR) products was used. From an initial sample of 56 differential fragments, 8 candidate fragments were identified. After Northern blot analysis, we obtained 5 positive fragments. The result indicated that this method could quickly screen positive clones. It is faster, easier and more efficient. The method could be used not only to analyze the various products of the differential fragments, but also to check up the mutations of genes in gene diagnosis.
出处
《基础医学与临床》
CSCD
北大核心
2002年第3期278-280,共3页
Basic and Clinical Medicine
基金
国家自然科学基金 (39770 713)
