摘要
本文采用聚丙烯酰胺凝胶盘状电泳法分别分析了黄兔尾鼠(Lagurus lutcus Eversmann)和草原兔尾鼠(Lagurus lagurus Pallas)的八种组织器官(肝脏、心脏、肌肉、肾脏、胃、脑、肺和横膈)中的乳酸脱氢酶(LDH)和酯酶(EST)同工酶。实验结果表明,(1)在同种动物的不同组织中,同工酶谱带及活性表现不同,具组织特异性。(2)异种动物的相同组织中,每种同工酶的谱带和活性不同,具有种间特异性。(3)在异种动物中,EST同工酶的差异性大于LDH同工酶的差异性;因此,EST同工酶可作为区分亲缘关系相近物种的遗传指标。(4)黄免尾鼠似乎是通过增加LDH同工酶的B亚基和EST同工酶的活性来适应干旱及半干旱的荒漠生境。
L. luteus Eversmann and L. lagurus Pallas are main harmful rodents in grasslands and deserts of northern Xinjiang. For exploring the characteristics of biochemical genetics and adaptedness to the circumstances, the lactate dehydrogenase (LDH) and the esterase (EST)isozymes, from eight tissues, including liver, heart, skeletal muscle, kindey, stomach, lung, brain and diaphragm, in the two species were analysed with polyacrylamide gel disc electrophoresis. The experimental results obtained are as follows:
1. There were five bands of LDH isozyme and nine bands of EST isozyme in the different tissues of each species. The zymograms and enzyme activities of the two kinds of isozyme in the different tissues have distict differences, so LDH and EST isozymes express the tissue specificity.
2. The electorphroetic patterns of LDH and EST isozyme exhibit apparent differences in the same tissue of the two species and present a species-specific tissue distribution.
3. Between the two species, the differentiation of EST is more remarkable than that of LDH. It is suggested that the EST isozymic analysis may have the potentiality to be applicable for the phylogenetic interrelationship of different varieties in a genus.
4. Perhaps L. luteus Eversmann is adaptive to drought or half-drought desert habitats by increasing the proportion of B subunit of LDH and activities of EST isozyme.
出处
《新疆大学学报（自然科学版）》
CAS
1991年第4期89-94,共6页
Journal of Xinjiang University(Natural Science Edition)
关键词
兔尾鼠
乳酸脱氢酶
酯酶
同功酶
Lagurus luteus Eversmann
Lagurus naqurus Pallas
lactate dehydrogenase(LDH)
esterase (EST): polyacrylamide gel disc electrophoresis: biochemical genetics
