摘要
目的 :研究板蓝根F0 2 2 部位抗内毒素活性并初步探讨其作用机理。方法 :ELISA法检测内毒素刺激鼠腹腔巨噬细胞分泌肿瘤坏死因子α(TNF α)、白介素 6 (IL 6 )水平 ;内毒素致兔发热实验 ;内毒素致BCG增敏小鼠死亡实验。结果 :F0 2 2 与LPS同时或F0 2 2 提前 1h处理巨噬细胞 ,能显著性抑制上清中TNF α和IL 6水平。先给予F0 2 2 ,能拮抗内毒素所致家兔发热反应 ,显著延长BCG增敏小鼠受LPS攻击后的存活时间 ,并呈剂量依赖性。结论 :以上结果提示F0 2 2 对内毒素拮抗作用发生在LPS激活机体免疫系统之前 ,该过程F0 2 2
Objective: To study the anti endotoxin activity and mechanism of F 022 from Radix Isatidis. Method: The production of TNF αand IL 6 of murine peritoneal macrophages stimulated by LPS was measured by ELISA. The temperature in rabbits was tested after iv administration of LPS. The lethality of BCG primed mice was induced by LPS. Result: If F 022 was added to macrophages culture simultaneously with LPS or 1 h before addition of LPS, production of TNF αand IL 6 by macrophages was remarkably inhibited in vitro . F 022 inhibited the fever induced by LPS in rabbits and protected BCG primed mice from LPS induced lethality if given before administration of LPS. Conclusion: The anti endotoxin effect of F 022 may inhibit LPS binding to its receptor, and it may be a LPS receptor antagonist.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2002年第6期439-442,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目 (3 9870 872 )
