摘要
目的 :改良成人成骨细胞消化培养方法 ,研究阿伦磷酸钠对成人成骨细胞增殖的影响。方法 :手术中获取病人的髂骨块 ,酶消化法得到成骨细胞 ,传 4代后以 1× 10 5/ml的浓度种于 2块 96孔板中。MTT方法检测阿伦酸钠对成骨细胞增殖的影响。结果 :高于 10 4M浓度的阿伦磷酸钠明显抑制成骨细胞的增殖 ,浓度低于 10 5M时 ,成骨细胞的增殖不受影响。结论
Objective:Using modified digestive method to obtain and culture human osteoblast,and investigate the effect of alendronate on osteoblasts.Methods:Human ilium cancellous bone was harvested in surgery and digested by trypsin and collagenase of 30 mins to remove blood cells and fibroblast.Bone chips were digested again for l h,a large amount of pure osteoblasts were released and used for research.After 4 generation subculture,osteoblasts were seeded in 96-well plates at the density of 1×10 5/ml.24h later,10 7 M～10 3 M alendronate culture medium were changed.MMT method was used to assay proliferation of osteoblasts.Results:≥10 4 M alendronate inhibited proliferation of osteoblast;≤10 5 M alendronate has no effect on osteoblastic proliferation.Conclusion:The modified digestion method could obtain nearly pure human osteoblast.High concentrated alendronate inhibited osteoblastic proliferation.
出处
《中国矫形外科杂志》
CAS
CSCD
2002年第5期464-466,共3页
Orthopedic Journal of China
关键词
阿伦磷酸钠
成骨细胞
增殖
Alendronate
Human Osteoblasts
Proliferation
