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酵母双杂交系统及发展

The yeast two-hybrid system and its development
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摘要 酵母双杂交系统是一种用来在体内研究蛋白质之间相互作用的一个非常有效的手段 ,并且已经得到了广泛的应用 ,它包括双杂交、反向双杂交和三杂交系统等 . The yeast two hybrid system is a powerful technique for detecting in vivo protein interaction and becoming wildly used in the function study of protein. It includes two hybrid,reverse two hybrid and three hybrid system.Here a brief elucidation of the principle and the application of the yeast two hybrid system is presented.
出处 《周口师范高等专科学校学报》 2002年第2期63-66,共4页 Journal of Zhoukou Teachers College
关键词 酵母 双杂交系统 发展 反向双杂交系统 三杂交系统 蛋白质 yeast two hybrid system reverse two hybrid system three hybrid system protein
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  • 1Ma J, Ptashne M. A new class of transcriptional activators [J ]. Cell, 1987,51 : 113 - 119
  • 2Fields S, Song O. A novel genetic system to d erect protein - protein interactions[ J ]. Nature, 1989,340 : 245 - 246.
  • 3James P. Halladay J .Craig E A. Genomic libraries and a host strain designed for highly efficient tow- hybrid selection in yeast[ J ]. Genetics. 1996.144 : 1425 - 1436.
  • 4Kasten M M.Ayer D E,Stlilman D J. SIN3 - dependent transcriptional repression by interaction with the Madl DNA -blinding protein[ J ]. Mol Cell Biol, 1996.16:4215 - 4221.
  • 5Frederickson R M. Macromolecular matchmaking: Advances in two- hybrid mid related teclmologies[J ]. Curt Opin Biotechnol, 1998,9 :90 - 97.
  • 6Grawunder U,Wilm M,Xiaotuo W.et al. Activity of DNA ligase IU stimulated by complex formation with XRCC4 prorein in mammalian cells[J], nature. 1997. 388 ;492 - 495.
  • 7Kkston J, Ravsn T, Yingping J, et al. A deathdomain - containing receptor that mediates apoprosis[ J ]. Nature, 1996,384 :372 - 375.
  • 8Melegarim M,Scaglionip P,Wands J R. Cloning and characterization of a novel hepatitis B virus X binding protein that inhibits viral replication[J] .J Virol,1998,72,1737 - 1743.
  • 9Vidal M, Braehmann R K,Fattaey A, et al. Reverse two- hybrid and tree- hybrid system to detect dissociation of protein and protein- DNA interaetions [J]. Proc Natl Acad Sci USA, 1996,93 : 10315 - 10320.
  • 10Vidal M,Braun P, Chen E, et al. Genetic characterization of a manmalian protein- protein interaction domain by using a yeast reverse two - hybrid system[J]. Proc Natl Acad Soi USA,1996,93:10321 - 10326.

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