摘要
目的:观察冷冻保存对羊膜超微结构的影响,为临床应用提供实验依据。方法:将新鲜制备的羊膜和-80℃冷冻保存30d、60d、90d、180d的羊膜分别进行透射电镜检查,观察其超微结构的变化。结果:新鲜制备的羊膜上皮细胞结构完整,胞浆内细胞器丰富,细胞间可见桥粒连接,基质中可见大量的胶原微纤维,并可见明暗相间的纤维横纹;随着冷冻保存时间的延长,羊膜上皮细胞逐渐变性死亡,冷冻保存90d时,上皮细胞染色质溶解,胞浆内细胞器变性,线粒体空化;冷冻30d和60d时,基质胶原微纤维形态与新鲜制备的羊膜相似;冷冻90d时,基质中部分胶原微纤维肿胀;冷冻180d时,局部胶原微纤维肿胀溶解。结论:冷冻保存可致羊膜上皮细胞死亡和基质胶原微纤维肿胀溶解。眼科学报2001;17:202～205。
Objective: To observe the effect of frozen storage for amniotic membrane ultrastructure, and provide the experimental evidence for clinical use.Methods: The amniotic membranes of fresh-obtained and - 80℃ frozen storage 30 d, 60 d, 90 d and 180 d were examined with transmission electron microscope, and the ultrastructural changes were observed.Results: The epithelium in the fresh-obtained amniotic membrane was observed as intact structure, abundant organelles in cytoplasm and intercellular contact with desmosome. A great quantity of collagen micro-fibrils were found in the stroma, and the light and shade striation were observed in the collagen micro-fibrils of fresh-obtained amniotic membrane. The epithelium degenerated and died gradually as the increase of frozen storage time. At the frozen storage of 90 days, the epithelial chromatin dissolved, organelles degenerated and mitochondrion vacuolated. At the frozen storage of 30 days and 60 days, the pattern of collagen micro-fibrils in stoma was similar to that in fresh-obtained amniotic membrane; At the frozen storage of 90 days, some of collagen micro-fibrils in stoma showed intumescence. At the frozen storage of 180 days, the number of collagen micro-fibrils in stoma decreased, and some of them showed intumescence and dissolvent.Conclusion: Frozen storage can result in amniotic membrane epithelium death and collagen micro-fibrils intumescence and dissolvent. Eye Science 2001; 17: 202 ~ 205.
出处
《眼科学报》
2001年第4期202-205,216,共5页
Eye Science
基金
上海市科学技术发展基金资助
项目编号:994119008
