摘要
目的:探讨联合羊膜的小片状同种异体结膜移植重建眼表的可行性。方法:新西兰白兔双眼上方球结膜碱烧伤后24h,实验组行以羊膜为基底的小片状同种异体结膜移植,对照组行单纯小片状同种异体结膜移植,术后各周行印迹细胞的PAS染色、扫描电镜检查。结果:实验组未见明显排斥反应,全部植片成活,对照组7眼植片排斥脱落,植片成活率81.6%,明显低于实验组(P<0.05)。实验组术后4wk,对照组术后第5wk结膜外观、杯状细胞形态、数目、功能均恢复正常。结论:羊膜与小片状同种异体结膜移植相结合可能成为修补早期大面积结膜碱烧伤的有效途径。
AIM： To explore the feasibility of combining small pieces of allo-conjunctiva transplantation （ACT） with amniotic membrane transplantation （AMT） to reconstruct ocular surface.
METHODS： Bilateral superior bulbar conjunctiva of New Zealand white rabbits were burned by NaOH （1mol/L）.After 24h, the right eye （experimental groups Exps） was performed AMT and ACT; the left eye （control groups Cons） was done merely ACT. The viability and function of allografts were identified by PAS staining and scanning electron microscope （SEM） of cytology impression （CI） every week after the operation.
RESULTS： In Exps, no obvious rejection was observed. All grafts survived, extended and covered the conjunctival defect. The appearance of grafts, and the shape, number, and function of goblet cells recovered perfectly four weeks after the operation; however, in Cons, obvious rejection was found. Seven grafts were rejected and. symblepharon was observed in those eyes. The appearance of survival allografts were similar to the normal conjunctiva, with similar shape, number and function of goblet cells till the fifth week after the operation. The survival ratet was significantly higher in Exps than in Cons （P 〈0.05）.
CONCLUSION： ACT based on AMT can be considered a good way to reconstruct ocular surface for large area conjunctiva alkali burns in early-stage.
出处
《国际眼科杂志》
CAS
2005年第6期1093-1096,共4页
International Eye Science
关键词
同种异体结膜移植
羊膜移植
印迹细胞检查
allogenic conjunctiva transplantation
amniotic membrane transplantation
cytology impression
