摘要
目的 :探讨赖型钩体弱毒株外膜蛋白基因的特点及其与强毒株的差别。方法 :对问号钩体赖型 5 660 1株的外膜蛋白基因进行PCR扩增得到外膜蛋白基因并进行了全基因序列测定 ,并与我国特有的强毒株 0 17株进行了Omp11基因核苷酸序列、推导的氨基酸序列以及亲水性和疏水性和二级结构的比较。结果 :赖型钩体 5 660 1株和 0 17株Ompl1基因核苷酸序列及推导的氨基酸序列同源性均为 99%。结论 :赖型钩体 5 660 1株和 0 17株Ompl1基因核苷酸序列存在较小差别 ,可能与毒力强弱有关。另外 ,两株钩体Ompl1基因的核苷酸序列及推导的氨基酸序列同源性很高 。
Objective: To study the difference of Omp11 gene of two strains of L. interrogans serovar lai.Methods: The Omp11 genes of strain 56601 were obtained by PCR and the sequence analysis was conducted. The nucleotide sequence, deduced amino acid sequence, hydrophilicity and secondary structure were compared with strain 017, Results: The sequences of the Omp11 gene and deduced Omp11 protein sequences of strains 56601 and 017 showed an identity of 99%.Conclusion: The sequences of Omp11 gene have little difference between both strains and vaccine can protect against the wild strains.
出处
《西南国防医药》
CAS
2002年第2期107-109,共3页
Medical Journal of National Defending Forces in Southwest China
