摘要
绿秆芋茎尖可在MS+0.2mg/l NAA+4mg/l BA+2mg/lABA+20g/l 甘露醇的培养基上,于15℃、100lx 光照的条件下保存350天,单芽存活率达90%以上,且具良好的生长状态与恢复分化的能力。以芽的叶柄为外植体,在MS+0.2mg/l NAA+4mg/l BA 培养基中培养,一代繁殖系数可达5.5以上。
The shoot tips of Green Stem taro(Colocasia esculenta Schott var.cormosa Chang),a common cultivar in China,were cultured in different conditions.The best result of their invitro preservation was obtained on the medium of“MS+0.2mg/1 NAA+4mg/1BA+2mg/1 ABA+20g/1 Mannitol”at 15℃ and 1000 lux of light.After 350 days of invitro culture,over 90% of the explants survived with satisfactory potential of growth anddifferentiation.Cultured on the medium of“MS+0.2mg/1 NAA+4mg/1 BA”,the petiolcsof buds rctained high propagation coefficient(above 5.5 per generation).
出处
《西南农业大学学报（自然科学版）》
CSCD
1991年第4期409-412,共4页
Journal of Southwest Agricultural University
基金
四川省应用基础研究基金
关键词
芋头
试管芽
保存
Colocasia esculenta Sehott var.cormose Chang
bud
preservation
/tube
