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人VEGF受体II基因3区的克隆、表达研究 被引量：5

Cloning and Expression of Domain3 of VEGF Receptor II
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摘要 用RT PCR法从人胎儿脐静脉内皮细胞中克隆人VEGF受体II基因 3区 ,将其重组到 pAYZ表达载体中 ,构建成人VEGF受体II基因 3区表达载体。将该载体转化大肠杆菌 16C9,获得稳定表达 ,表达产物以可溶性状态存在 ;SDS PAGE和Westernblot分析显示 ,其分子量约为 14kD。以纯化的表达产物免疫Balb/c小鼠 ,眼球采血制备抗血清 ,抗血清效价在 10 3以上 ,并具有抑制VEGF诱导内皮细胞增殖的作用 ,在抑制肿瘤血管新生中具有潜在的应用前景。 RT PCR was used to clone DNA fragment of domain3 of VEGF receptor II (KDRIII ) from human umbilical vein endothelial cell, and the expression vector of KDRIII was constructed by cloning KDRIII DNA fragment into the expression vector pAYZ KDRIII was expressed in E coli 16C9 and purified by affinity chromatography The serum against KDRIII was prepared from mice immunized with a soluble form of KDRIII The serum against KDRIII suppressed proliferation of ECV307 induced by VEGF and have potential clinical application in the treatment of cancer where pathological angiogenesis is involved
作者 熊冬生 刘嘉 邵晓枫 李蓉 许元富 彭晖 范冬梅 朱祯平 杨纯正
机构地区 中国医学科学院中国协和医科大学血液学研究所实验血液学国家重点实验室
出处 《高技术通讯》 EI CAS CSCD 2002年第5期45-49,共5页 Chinese High Technology Letters
基金 国家攀登计划 (95 专 10 ) 天津市重大科技攻关 (0 0 3 1195 11)资助项目
关键词 VEGF受体 Ⅱ基因3区 血管内皮细胞生长因子 血管内皮细胞生长因子受体 人体 血管新生 肿瘤治疗 基因克隆 基因表达 VEGF, KDR, Cloning, Expression
分类号 R73-36 [医药卫生—肿瘤]
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共引文献17

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高技术通讯
2002年 第5期

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