摘要
首次克隆了小鼠神经元标志性微管蛋白 βⅢ基因 ,从核苷酸序列推导出小鼠与人两者之间在其羧基端有相同的EAQGPK六肽 ,进一步证实用抗人微管蛋白 βⅢ单抗可检测小鼠神经干细胞分化成的神经元细胞 .免疫组化鉴定显示小鼠神经干细胞在体积分数为 1%胎牛血清 (FBS)诱导下 ,可分化成神经元、星形胶质细胞、少突胶质细胞 .同时培养了 13周龄胎儿脑来源的人类神经干细胞 ,用特异性的抗人nestin抗体鉴定 ,全部为阳性细胞 ,但是它们经诱导分化产生较不同寻常的细胞分化结果和分化程度 ,在生长因子减半和 1%FBS诱导条件下可分化为神经元和星形胶质细胞 ,而无少突胶质细胞分化 。
A mouse neuron specific gene tubulin beta 3 has been cloned for the first time. It is found that there is the same epitope composed of six amino acids at the carboxyl terminal between human and mouse protein which was deduced from the nucleotide sequence of mouse tubulin beta 3. Thus, it is confirmed that mouse derived neurons can be detected with mouse anti human tubulin beta-3 monoclonal antibody.Mouse neural stem cells can differentiate into neurons, astrocytes and oligodendrocytes in vitro under the induction of 1% fetal bovine serum (FBS). Meanwhile, human neural stem cells from 13 week gestation forebrain tissue have been cultured successfully, all neural stem cells are positive stained by human specific anti nestin polyclonal antibody. When induced, they show the unusual results. With 1% FBS and half dose of two growth factors(EGF and bFGF), they can differentiate into neurons and astrocytes, but no oligodendrocytes. Further, the results of anti neurofilament monoclonal antibody assay show the differentiated neurons still stay in the early developing stage.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2002年第1期57-62,共6页
Journal of Fudan University：Natural Science
基金
国家"攀登"计划资助项目
关键词
微管蛋白βⅢ
体外分化
神经干细胞
tubulin β Ⅲ
differentiation in vitro
neural stem cells
