摘要
目的 研究抗病毒蛋白ＭｘＡ的表达及其活性。方法用ＩＦＮα２ｂ或３型腺病毒（Ａ＿３）分别对ＷＩ－３８细胞或人外周血单个核细胞（ＰＢＭＣｓ）作用 １２或２４ ｈ，并用Ｗｅｓｔｅｍ ｂｌｏｔ法或ＦＡＣＳ法，分别对Ｍ蛋白的表达进行定性和定量检测。采用微量细胞病变抑制法，对重组的ＭｘＡ和ＩＦＮα２ｂ进行抗病毒效应实验。结果 （１）低浓度的 ＩＦＮα２ｂ（＞ １×1０～＃ＩＵ／Ｌ）和Ａｄ＿３（＞２００ ＴＣＩＤ＿５０），均可诱导相应细胞表达 ＭｘＡ；（２）１０μｇ／Ｌ ＭｘＡ可抵抗２０个 ＴＣＩＤ＿（５０）的 ＨＳＶ－Ｉ、Ｐｏｌｉｏ． Ｖ感染 Ｖｅｒｏ细胞、Ａｄ＿３感染ＨｅＬａ细胞，抵抗２００个ＴＣＩＤ＿（５０）的ＶＳＶ感染Ｗｉｓｈ细胞。结论ＭｘＡ只能由干扰素（ＩＮＦα2ｂ）或病毒诱导产生，其具有广谱的抗病毒活性。
Aim To study the expression and activity of antivirus protein MxA. Methods WI-38 cells or human PBMCs were trea- ted by IFNα 2b or adenovirus Ad~ 3 for 12 or 24 hous. The expression of MxA protein were detected by Western blot or FACS. Antiviral effect of rhMxA and IFNα2b were tested by micro cytopathogenic effect inhibition assay. Results (1) Low concentration of IFNcα2b （> 1 × 103 IU/L) and Ad_3 ( >200 TCID_(50)) could induce expres- sion of MxA protein in WI-38 cells or human PBMCs. (2) rhMxA protein 10μg/Lcould protect Vero, HeLa and Wish cells from 20 TCID_50）HSV-I, poliovirus Ad_3 and 200 TCID_(50) VSV. Conclusion MxA protein can be induced only by interferon (IFNα2b) or virus and has broad-Spectr antiviral effect in vitro.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期77-79,共3页
Chinese Journal of Cellular and Molecular Immunology
