摘要
目的 :比较肿瘤内直接注射3 2 P胶体和先向肿瘤内注入MAA再注射3 2 P胶体两种给药方法的3 2 P的体内分布及其治疗作用。方法 :6 0只荷肿瘤小鼠随机分为 4组 ,第 1组只注射3 2 P胶体 1.85MBq ;第 2组先注入 1× 10 4 颗粒的MAA ,再注入3 2 P胶体 1.85MBq ;第 3组先注入 1× 10 5颗粒MAA ,再注入3 2 P胶体 1.85MBq ;第 4组先注入 1× 10 5颗粒MAA ,再注入3 2 P胶体 18.5MBq。注射后第 30min、2 4h、4 8h、8d和 16d ,从各组随机取出 3只小鼠 ,测定肿瘤直径 ,采血后处死 ,取出肿瘤、心、肝、脾、肾、肺和脊椎骨 ,称重并计数放射性。对第 4组小鼠的肿瘤做病理切片并计算抑瘤率。结果 :3 2 P自肿瘤向血液中的扩散主要发生在用药后的早期阶段 ,MAA可以阻止3 2 P的扩散 ,增加其瘤内滞留率。MAA的阻滞作用与注入的MAA颗粒数量成正相关 ,而与3 2 P胶体的注入量则成负相关关系。对于直径约 1cm的肝肿瘤 ,注入 1.85MBq的3 2 P胶体不能抑制肿瘤的生长 ,18.5MBq3 2 P胶体可使肿瘤缩小 2 0 % ,对外周血红细胞和白细胞计数无明显影响作用。结论 :直接向肿瘤内注入一定数量的MAA ,再注入所需剂量的3 2 P胶体 ,是治疗晚期肝癌安全有效的方法。
Objective: To investigate the tumor deposition,systemic biodistribution and therapeutic effect of colloidal 32 P in different intratumoral injection methods. Method: H 22 hepatocellular cancer cells were inoculated subcutaneously in 60 male Balb/c mice, which were divided into four equal groups randomly when the tumors reached 1.0 cm in diameter. 1.85MBq/0.1ml of colloidal 32 P were injected slowly into the tumors in one group. 1×10 4 particles of MAA/ 0.1 ml followed by 1.85 MBq/0.1ml of colloidal 32 P was injected in another group, 1×10 5 particles of MAA/0.1ml followed by 1.85 MBq/0.1ml colloidal 32 P in the third group, and 1×10 5 particles of MAA/0.1ml followed by 18.5MBq/0.25ml colloidal 32 P in the fourth. Three mice from each group were sacrificed at 30min, 24h, 48h, 8d and 16d after injection. A sample of 100μl blood was collected, the whole tumor, heart, liver, spleen, kidneys, lungs and a piece of backbone of each animal were removed, weighted, and counted for radioactivity, the diameters of the tumors were measured and the pathological results were examined. Result: The diffusion of colloidal 32 P from tumor to blood occurred mainly in the early stage after inject of MAA particles number led to a better retention of colloidal 32 P in tumors, but when the number of MAA particles was same, the obstructive effectiveness decreased with increased doses of colloidal 32 P. For tumors with a diameter of 1.0 cm, a dose of 18.5 MBq colloidal 32 P was found to control the tumor growth with no bone marrow suppression. Conclusion: It is a safe and effective therapy for unresectable hepatic cancer to inject intratumorally a certain number of MAA particles followed by a proper dose of colloidal 32 P.
出处
《山东大学学报（医学版）》
CAS
2002年第2期160-162,167,共4页
Journal of Shandong University:Health Sciences
