摘要
目的 :对含有P2 2、P30复合基因的重组菌进行IPTG诱导表达 ,进而检测复合基因的融合表达产物的免疫活性。方法 :用IPTG对工程菌进行诱导表达 ,表达产物行SDS PAGE蛋白凝胶电泳及Western Blot免疫印记检测。结果 :蛋白电泳结果显示 ,阳性重组菌比阴性菌在 6 6 .5KDa位置上明显多一条带 ,此条带可与P30抗体结合并使免疫印记显示阳性结果。结论 :含有P2 2、P30基因片段的质粒重组体经诱导表达出融合蛋白 ,其内的组分蛋白P30具有与其相应抗体结合的能力。
Objective: To induce the engineering bacteria E.coli JM109 containing the recombinant plasmid ,and check up the activity of the expression products. Methods:Expression of the engineering bacteria was induced by IPTG, and the expression products were identified by SDS PAGE and Western Blot analysis. Results: The positive recombinant bacteria had one more protein band at 66.5 Kda site compared with the negative control. Western Blot with p30 antibody showed there was a brown band at the same site. Conclusion: Expression of P22 P30 fusion protein was successfully induced by IPTG, and it can be combined by P30 antibody.
出处
《山东大学学报（医学版）》
CAS
2002年第2期123-124,127,共3页
Journal of Shandong University:Health Sciences
基金
山东省卫生厅科研基金资助课题 ( 99CA1CAA10 )
