摘要
目的 选择简单的方法筛选“渗漏”型SCID小鼠。 方法 采用 3种酶联免疫吸附法 (双抗夹心ELISA、双抗夹心间接ELISA及生物素 亲和素夹心ELISA)测定SCID小鼠血清中IgG含量。 结果 生物素 亲和素夹心ELISA法灵敏度最高 ,用此法共检测了 4 1只 6～ 8周龄小鼠 ,其中 4 0只的IgG含量为 0 2 4± 0 16mg L ;只有 1只小鼠IgG含量为 2 3mg L ,渗漏比例为 2 4 %。 结论 用生物素 亲和素夹心ELISA法对SCID小鼠“渗漏”进行初筛 ,是简便、敏感。
Objective Choose an easy method to screen the “leaky' phenotype SCID mice before experimental use. Methods IgG in SCID mice serum was assayed by three kinds of ELISA(sandwich ELISA, sandwich indirect ELISA and Avidin\|biotin sandwich ELISA). Results Avidin\|biotin sandwich ELISA is the most sensitive method of the three, and 41 SCID mice of 6～8 weeks old were assayed with this method. The average IgG of 40 SCID mice was 0 24±0 16mg/L, only one was 2\^3mg/L. The “Leaky' probability was 2\^4%.Conclusion\ The Avidin\|Biotin sandwich ELISA is an easy, sensitive and reliable method to screen“leaky' SCID mice.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期111-112,共2页
Acta Anatomica Sinica
关键词
渗漏
酶联免疫吸附法
SCID小鼠
Leaky
Enzymelinked immunosorbant assay (ELISA)
SCID mice
