摘要
为探讨先天性Ｔ－Ｂ淋巴细胞联合缺陷对生殖功能的影响，用正常ＢＡＬＢ／ｃ小鼠作对照，对重度联合免疫缺陷（ＳＣＩＤ）小鼠的排卵、胚胎（胎儿）发育能力及妊娠母体外周血浆促黄体素（ＬＨ）和孕酮（Ｐ）水平的变化进行了研究。首先，观察了妊娠期、窝产仔数，发现ＳＣＩＤ小鼠大多在妊娠第２０天（检栓当天为第１天）凌晨分娩，平均窝产仔数为（３．８６±１．６７）只（ｎ＝４２），ＢＡＬＢ／ｃ小鼠多在妊娠第２０天较晚或第２１天凌晨分娩，平均窝产仔数为（４．４４±１．５４）只（ｎ＝２０），比ＳＣＩＤ小鼠约多０．６只。取１０周龄ＳＣＩＤ雌鼠４０只，ＢＡＬＢ／ｃ雌鼠５０只，分别于第２、６、１０、１５、１９（ＳＣＩＤ）或２０（ＢＡＬＢ／ｃ）天早晨各取５～１０只用于试验。结果表明：（１）ＳＣＩＤ小鼠平均排卵数比ＢＡＬＢ／ｃ小鼠少约０．７个（分别为８．００±１．９０和８．６６±２．２６），而产前１ｄ活胎数占排卵数的百分比在ＳＣＩＤ小鼠和ＢＡＬＢ／ｃ小鼠间无差异（分别为６６．４７％和６６．３８％），说明ＳＣＩＤ小鼠产仔数比ＢＡＬＢ／ｃ小鼠产仔数低是由于排卵数少造成的；（２）ＳＣＩＤ小鼠在妊娠最后５ｄ，外周血浆水平下降迅速，这期间胎儿死亡率高；ＢＡ?
The effects of severe combined imunodeficiency on reproduction have been determined in the present experiments by studying ovulation, embryonic development and changes in plasma concentrations of LH and P during pregnancy in SCID mice. First of all, we have observed the length of gestation and litter size and found that most of the pregnant SCID mice delivered before dawns on d20(the day the plug was found was designated d1 of pregnancy) with a litter size of 3.68±1.67,whereas the BALB/c mice gave birth to (4.44±1.54)babies in the late on d20 or before dawn on d21 of pregnancy. Forty to fifty of 10 week old female SCID and BALB/c mice respectively were used in the following experiments. Pregnant mice on d2, d6, d10, d15 and d19(SCID) or d20 (BALB/c) were exsanguinated from the intra orbital sinus and then killed and embryos/fetuses were examined. Concentrations of plasma LH and P were assayed. Results showed that: 1) ovulation rate of SCID and BALB/c mice were 8.00±1.90 and 8.66±2.26 respectively, the difference was about 0.7 which equals approximately to the difference of litter size (0.6), while the percentage of live fetuses to ovulated ova(counted by number of corpus lutea) on one day before parturition were the same(66.47% and 66.38%, respectively), suggesting that the difference of litter size was caused by the difference of ovulation rate.2) fetal mortality in SCID mice was the highest during the last 5 days of pregnancy when concentration of P decreased rapidly. But fetal mortality in BALB/c was the highest and concentration of P decreased greatly during d10 to d15.3) There was no significant difference in concentrations of LH during pregnancy between SCID and BALB/c mice, however, all of the highest level(d15, (61.01±25.25)nmol/L), the lowest level (d2, (5.84±4.84)nmol/L) and average level during pregnancy (( 31.69 ± 22.24 )nmol/L) of P in SCID mice were lower than those in BALB/c mice (d10, (79.80±27.90)nmol/L; d2, (13.69±9.92)nmol/L and (44.75±21.46)nmol/L, respectivly). Thus we conclude that severe combined immunodeficiency affects ovarian events related to ovulation and steroid secretion in mouse.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第6期602-605,共4页
Chinese Journal of Veterinary Science
基金
军队医药卫生科研基金
关键词
小鼠
SCID
排卵
妊娠
促黄体素
孕酮
mouse
severe combined immunodeficiency, SCID, ovulation
pregnancy
luteinizing hormone
progesterone
