摘要
目的 研究 5 羟色胺 (5 HT)样免疫反应纤维终末与大鼠三叉神经本体觉中枢通路二级神经元之间是否存在突触联系。 方法 逆行束路追踪与免疫组织化学相结合的电镜双重标记技术。 结果 将麦芽凝集素结合的辣根过氧化物酶 (WGA HRP)注入大鼠三叉神经感觉主核背内侧部 (Vpdm)并进行 5 HT免疫染色后 ,在三叉神经脊束核吻侧亚核背内侧部及其邻接的外侧网状结构 (Vodm LRF)中可见WGA HRP逆行标记的神经元和 5 HT样阳性轴突终末。电镜下观察到 5 HT样阳性轴突终末与WGA HRP标记的神经元之间有轴 体、轴 树突触联系 ,这些突触属对称或非对称型 ,但以对称型为主。 结论 本研究为 5
Objective To investigate whether the serotonin(5\|HT)\|like immunoreactive axons synapse upon the second\|order neurons on the central pathway of the trigeminal proprioception in the rat. Methods Electron microscopic double\|labeling of retrograde tract\|tracing technique combined with immunohistochemistry. Results Following the injection of wheat germ agglutinin\|horseradish peroxidase (WGA\|HRP) into the dorsomedial part of the principal sensory trigeminal nucleus(Vpdm) of the rat and the immunostaining for 5\|HT, there were neurons retrogradely labeled by WGA\|HRP and axon terminals with 5\|HT\|like immunoreactivity(5\|HT\|LI) in the dorsomedial part of the subnucleus oralis of the spinal trigeminal nucleus and its adjacent lateral reticular formation(Vodm\|LRF).At the electron microscopic level,the 5\|HT\|immunostained axonal profiles were observed to make synaptic contacts with the WGA\|HRP\|labeled neurons.Both the axon\|somatic and axon\|dendritic synapses were found.These synapses were mainly of symmetric type although the asymmetric ones were also seen.Conclusion\ The present results provided a morphologic basis for the serotoninergic terminals which might be involved in the regulatory effect for the trigeminal proprioceptive information transmission.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期11-15,共5页
Acta Anatomica Sinica
基金
ThePresentworkwassupportedbyNationalNaturalScienceFoundationofChina(3 9870 2 62 )andbyFoundationforUniversityKeyTeacherbytheMinistryofEducation .
