摘要
目的 探讨B淋巴细胞在免疫相关性全血细胞减少症 (IRP)发病中的作用。方法 采用流式细胞术测定 2 5例初诊患者 ,1 5例治疗后血常规恢复正常的患者和 1 0名正常人骨髓总B淋巴细胞、CD5+ B淋巴细胞的数量以及B淋巴细胞膜表面凋亡相关蛋白Fas抗原 (CD95)、胞浆内抗凋亡蛋白bcl 2的表达。结果 IRP初诊患者总B淋巴细胞、CD5+ B淋巴细胞比例显著高于治疗后血常规恢复组和正常人 (P均 <0 .0 5) ,后两组相比 ,差异无显著性 (P >0 .0 5) ;三组B淋巴细胞Fas抗原的表达率差异无显著性 (P均 >0 .0 5) ;初诊患者B淋巴细胞胞浆内bcl 2的表达率显著高于其它两组 (P均 <0 0 5) ,治疗后血常规恢复组也显著高于正常对照组 (P <0 .0 1 ) ;各组间B淋巴细胞凋亡相关指数(CD1 9+ CD95+ /CD1 9+ bcl 2 + )比较 ,初诊组显著低于治疗后血常规恢复组和对照组 (P均 <0 .0 1 ) ,治疗后血常规恢复组也显著低于对照组 (P <0 .0 5) ;B淋巴细胞的比例与治疗起效时间呈正相关 (r =0 .69,P <0 0 1 )。结论 IRP患者自身抗体的产生可能与B淋巴细胞及其亚群数量异常。
Objective To examine the quantity and apoptosis related protein level of B lymphocyte in the patients with imunorelated pancytopenia(IRP)and explore the role of B lymphocyte in the pathogenetic mechanism of IRP. Methods Quantities of all B lymphocytes and CD 5 +B lymphocytes and the expressions of Fas and bcl 2 on B lymphocytes in 25 patients with untreated IRP, 15 IRP patients in complete remission(CR) and 10 normal controls were assayed by FACS. Results The percentages of B lymphocyte and CD 5 +B lymphocytes were significantly higher in untreated IRP patients than in CR IRP patients and normal controls (P<0.05); there was no significant difference between the latter two groups (P>0.05). There was no significant difference of Fas expression in B lymphocytes among the three groups (P>0.05). The expression of bcl 2 on B lymphocytes was significantly higher in untreated patients than in CR patients or normal controls (P<0.05), and so did in CR patients than in normal controls (P<0.01). The apoptosis related index was significantly lower in untreated patients than in CR patients or normal controls (P<0.01), and was lower in CR patients than in normal controls (P<0.05). The percentage of B lymphocyte was positively correlated with the duration from the beginning of treatment to response. Conclusion The production of auto antibodies in IRP patients probably has some relationships with the abnormal quantities of B lymphocyte and its subsets, and with the inhibition of B lymphocyte apoptosis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2002年第5期236-238,共3页
Chinese Journal of Hematology
