摘要
目的观察软骨细胞在松质骨骨基质明胶(bonematrixgelatin,BMG)上生长分化的状况。方法分离幼兔关节软骨细胞,以松质骨BMG为支架材料体外培养,于不同时间取材行组织学观察。结果软骨细胞在松质骨BMG表面及内部孔穴能较好分裂增殖,软骨细胞周围基质Safranin-o及胶原染色阳性。培养到24～42d形成直径约4mm的“圆盘状软骨”。结论软骨细胞在松质骨BMG上体外培养,能形成软骨组织。
Objective To observe growth and differentiation of chondrocytes cultured in cancellous bone matrix gelatin(BMG).Method Chondrocytes were isolated from rabbit articular cartilage, and cultured in cancellous BMG in vitro.The histomorphology was observed at different interval time. Result Chondrocytes were found to be proliferating in and around the pores of cancellous BMG ,and the safranin-O and collagen staining were positive in the matrix around chondrocytes.'The cartilage discs' were formed from 24 days to 42 days.Conclusion Chondrocytes were cultured in Cancellous BMG in vitro. The cartilage-like tissues were formed.
出处
《中国临床康复》
CAS
CSCD
2002年第2期188-188,共1页
Chinese Journal of Clinical Rehabilitation
基金
陕西省自然科学基金资助项目(99SM47)
