摘要
系统研究了反应介质、水活度、温度、pH等因素对脂肪酶Novozym435催化乙酸甲基苯甲酯立体选择性氨解反应的影响。以正己烷为反应介质,酶表现出较高的催化活性和对映体选择性;适宜的反应温度为25℃～35℃;最适反应体系初始水活度为0.33;较适宜的pH范围为6～7。
The effects of reaction media, water activity, temperature and pH on Novozym 435-catalyzed enantiose-lective ammonolysis of (±) -α-methylbenzyl acetate have been systematically explored. Novozym 435 showed high catalytic activity and enantioselectivity in hexane; the optimum temperature and the initial water activity were 25℃ and 0.33 respectively; The suitable reaction pH was in the range of 6.0 - 7.0.
出处
《微生物学通报》
CAS
CSCD
北大核心
2002年第1期35-40,共6页
Microbiology China
基金
广东省自然科学基金资助项目(No.980543)
