摘要
探讨了超声对无溶剂体系中固定化脂肪酶Novozym 435催化废油脂转酯反应的促进作用,研究了超声功率、超声处理方式等对酶反应速度及酶操作稳定性的影响.结果表明:对酶进行适宜的超声预处理(20 kHz,80 W,30m in)能显著提高Novozym 435催化废油脂转酯反应的速度;经超声预处理的Novozym 435有良好的操作稳定性,反应10批次后,酶活下降甚微.
Aiming at the Novozym 435-catalyzed transesterification of waste oil in a solvent-free system under ultrasonic irradiation, the effects of ultrasonic power and radiation mode on the reaction rate and the operation stability were investigated. It is found that the reaction rate of Novozym 435-catalyzed transesterification of waste oil is remarkably accelerated by pretreatment of enzyme with ultrasonic irradiation at 20 kHz, 80 W for 30 min, and that Novozym 435 pretreated with ultrasonic irradiation is of good operation stability because its activity weakens little even after 10 batch operations.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2006年第5期68-71,共4页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省科技计划项目(2003C33102)
广州市科技计划项目(2005Z3-D0471)
关键词
超声辐照
脂肪酶
废油脂
转酯反应
ultrasonic irradiation
lipase
waste oil
transesterification
