摘要
目的 探索TH1/TH2平衡的体外诱导作用及其对嗜酸性粒细胞（Eos）功能的影响。方法 取人肝素抗凝外周全血,加入结核菌素纯蛋白衍生物（purified proteinderivative,PPD）10μg/mL,培养3、5、7d,用ELISA方法检测上清IL-4和r-IFN的含量。取3d上清培养液加入到肝素抗凝全血中,体外培养72h,IL-5作为阳性对照。用流式细胞术分析CD16阴性细胞群体中CD69的表达和碘化丙啶（PI）染色情况。结果 应用PPD体外诱导全血产生较高水平的r-IFN,IL-4含量未测出;培养体系中加入PPD诱导上清液明显抑制了Eos和IL-5活化的Eos CD69的表达并诱导其凋亡。结论 PPD体外成功诱导TH1/TH2平衡的转换;PPD诱导上清液具有活化Eos和诱导其凋亡的作用。
ve To explore the effect of PPD-induced whole blood supernatant on eosinophil apoptosis and activation( CD69 ex-pression) . Methods Human whole blood was diluted(l : 10)with IMDM culture medium and incubated for 3,5,7 d with PPD activation. Cytokines in the supernatant was detected by ELISA. 3 d PPD-induced supernatant was used to explore its effect on eosinophil apoptosis and ac-tivation. Whole blood was co-cultured with PPD-induced supernatant for 72 h ,and then CD16-PI and CD16- CD69+ cells were analyzed with flow cytometry. Results There was a high level ofr-IFN,but there was no detectable IL-4 in PPD-induced supernatant. 3 d supernatant sig-nificantly reduced CD16- CD69+ expression and induced the apoptosis of eosinophils and IL-5 activated eosinophils. Conclusions TH/TH2 profile switching was effectively induced by PPD in vitro. The results indicated that PPD possibly exert its effect by changing environment for eosinophil survival.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2002年第3期180-182,共3页
Immunological Journal
基金
国家自然科学基金(39800059)
广东省自然科学基金(980460)资助项目
