摘要
为探讨亚硒酸钠诱导人结肠癌SW480细胞凋亡的机理。将荧光探针2′,7′-二氯荧光黄乙二脂 (2′,7′-DCFH -DA)、罗丹明123(rhodamine123)负载人结肠癌细胞 ,利用多光子成像系统测定胞内活性氧 (ROS)、线粒体跨膜电位 (ΔΨm)的变化。结果发现 (1)Na2SeO3 作用SW480细胞 ,可导致细胞凋亡和胞内的ROS增加 ,SOD、过氧化氢酶可降低凋亡率并抑制ROS的增加。 (2)线粒体电子传递链抑制剂鱼藤酮及氰化钠可抑制ROS增加。 (3)Na2SeO3 可导致线粒体的跨膜电位的下降。表明Na2SeO3 作用细胞可导致来源于线粒体的ROS增加 。
To investigate the mechanism of apoptosis induced by sodium selenite in a human colonic cancer cell line SW480. SW480 cells were loaded with fluorescent probes 2′,7′- DCFH-DA, and rhodamine123, respectively. The changes of intracellular ROS, and mitochondrial transmembrane potential (ΔΨm) was detected by using multiphoton imaging system. The results showed that (1) Na2SeO3 elevated SW480 cells apoptotic rate and intracellular ROS; SOD, catalase evidently suppressed ROS increase and reduced apoptotic rate, (2) Inhibition of mitochondrial electron transport with NaCN or rotenone almost blocked selenite-induced ROS production, (3) Na2SeO3 induced disruption of the mitochondrial transmembrane potential (ΔΨm). The data suggest that Na2SeO3 results in the increase intracellular ROS which origitesfrom mitochondia and mediates Na2SeO3-induced apoptosis.
出处
《生物物理学报》
CAS
CSCD
北大核心
2002年第1期104-108,共5页
Acta Biophysica Sinica
基金
生物医学光子学教育部重点实验室开放课题资 (01)
