摘要
为研究 bcl- 2 / JH融合基因与鼻咽癌发生的关系 ,应用半套式原位 PCR技术对 41例石蜡包埋鼻咽癌组织中 bcl- 2 /JH融合基因进行检测。结果发现 41例癌组织中 bcl- 2 / JH融合基因阳性 6例 ,阳性率为 1 4 .6 % ,bcl- 2基因的两个热点断裂区 mbr和 m cr与免疫球蛋白重链 JH形成的融合基因 m br- JH和 m cr- JH各占 5 0 %。 bcl- 2 / JH融合基因形成与癌组织学分级和转移间无明显关系 (P>0 .0 5 )。本文表明 bcl- 2 / JH融合基因与鼻咽癌发生可能有一定关系 ,其中 m br- JH和 m cr- JH同为bcl- 2基因在鼻咽癌的两个重要基因异常改变形式。
To study the correlation between bcl 2/JH fusion gene and the occurrence of nasopharyngeal carcinoma (NPC), bcl 2/JH fusion gene in 41 cases of paraffin embedded NPC was investigated using semi nested in situ PCR technique. The results showed that bcl 2/JH fusion gene was present in 6 cases (14 6%), in which three were positive for mbr JH and three were positive for mcr JH. There were no significant differences in bcl 2/JH fusion gene formation between pathological grade and metastasis ( P >0 05). The result suggested that bcl 2/JH fusion might be involved in tumorigenesis of nasopharyngeal epithelium in which mbr JH and mcr /JH were the two important aberrant changes in NPC.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2000年第1期68-70,共3页
Chinese Journal of Histochemistry and Cytochemistry
