摘要
目的 探讨脂多糖（LPS）刺激的人血管内皮细胞（HUVECs）表面ICAM-1mRNA表达的规律以及高晶体-高胶体渗透压混合液（HHS）对此过程的影响。方法 分离健康产妇脐静脉内皮细胞,进行原代培养,细胞长至单层时用逆转录酶-聚合酶链反应法分析其ICAM-1mRNA的表达。结果 正常情况下HUVECs表面ICAM-1mRNA表达微弱,LPS刺激1h后表达开始增加,至4h处达到峰值。0.25％、0.5％高晶体-高胶体渗透压混合液均可明显抑制 LPS刺激的 ICAM-1mRNA表达（P<0.05）,但两者之间并无明显差异,且其作用时间对ICAM-1mRNA表达也无明显影响。结论LPS刺激的ICAM-1mRNA表达是一个缓慢而持续的过程。HHS可明显抑制脂多糖诱导的ICAM-1mRNA表达,且这种作用不受HHS浓度及作用时间的影响。
Objective To investigate the alteration in intercellular adhesion molecule (ICAM)-l mRNA expression on the surface of human vascular endothelial cells (HUVECs) induced by LPS stimulation and the effects of hypertonic-hyperoncotic solution (HHS) . Methods HUVECs were enzymatically isolated and cultured in RPMI 1640 culture medium at 37℃ for about 7 days when a monolayer of endothelial cells has grown. The primarily cultured HUVECs were mixed with LPS 100ng/ml and incubated at 37℃ for 30min, 1,4,8, 12and 24 h. 10%NaCl and 10% hydroxyethyl starch (Fresenius) were added to RPMI 1640culture medium. The HHS concentrations were 0.25% and 0.5% respectively. HUVECs were first incubated with either HHS (0.25% , 0.5% ) or isotonic solution for 10 min, 1h and 4h and then stimulated by LPS for 4h. The ICAM-1 mRNA was measured by reverse transcriptase-PCR. Results There was slight expression of ICAM-1 mRNA on the surface of HUVECs in normal condition. ICAM-1 mRNA expression began to increase at 1h exposure to LPS and reached peak value at 4h. Both 0.25% and 0.5% HHS could prevent the LPS-induced ICAM-1 mRNA up-regulation (P< 0.05), but there was no significant difference in the effect of HHS on ICAM-1 mRNA expression between the two concentrations. Moreover, the duration of HHS incubation with HUVECs had little effect on ICAM1 mRNA expression. Conclusions The LPS -induced ICAM-1 mRNA expression is slow and gradual. HHS could prevent the LPS induced ICAM-1 mRNA up-regulation which is irrelevant to its concentration and duration of pre-treatment.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2002年第5期283-286,共4页
Chinese Journal of Anesthesiology
