摘要
采用磷酸铅沉淀的电镜细胞化学方法 ,研究大豆种子萌发过程中 ,子叶细胞超微结构变化与ATP酶活性的消长。子叶细胞的ATP酶活性主要定位在质膜。在种子萌发的 0～ 3天 ,子叶细胞贮存蛋白质和油脂大量急剧降解 ,并快速转运到胚 ,质膜ATP酶活性较高。萌发的 6～ 9天 ,子叶细胞内含物大部分消解 ,蛋白体膜相互融合形成大液泡 ,原质体开始有稀疏的片层结构 ,ATP酶活性渐弱至无。萌发第 12天 ,原质体的片层堆叠成基粒 ,子叶细胞转为同化器官 ,制造养分 ,质膜再度呈现较强的ATP酶活性。
A comparative study on the cytochemical localization of ATP ase activity reaction in the cotyledon cells of soybean was carried out by electron microscopic observation of lead phosphate precipitation. ATP ase activity was mainly deposited in the plasma membrane. At 0～3 days after germination, the storage protein and lipid bodies decreased quickly and was transported to the embryo, the starch grain in the cells increased, and the plasma membrane was rich in ATP ase activity. At 6～9 days after germination, most of nutrients in the cell disappeared and the membrane of protein bodies coalesced with each other forming large centre vacuoles; in the proplastid there were undeveloped lamellae; ATP ase decreased and disappeared. At 12 days after germination the lamellae overal and granas appeared; the proplastids developed into chloroplasts; The cotyledonary was transforming from storage organon into assimilating organon; the ATP ase activity could reaction markedly increased on plasma membrane. It is suggested that the changes of ATP ase activity could reflect the physiological state of the cells.
出处
《电子显微学报》
CAS
CSCD
北大核心
2002年第2期114-117,共4页
Journal of Chinese Electron Microscopy Society
