摘要
从 10 0 0多份苎麻种质资源中筛选出抗旱性较强和较弱的栽培品种各 6个 ,选用 2 5个随机引物 ,对其总 DNA进行随机扩增。有 12个引物扩增得到了稳定的 RAPD图谱 ;扩增出的片段分子量在 0 .6～ 5 .15 kb之间 ,条带数在 3～ 15条之间 ,共扩增出 90个条带 ;采用聚类法中的中间距离法 ,对 12个品种的相似系数聚类生成树状图谱 ,分为两类三组 ,揭示了供试品种的亲缘关系 。
Six drought resistance and six sensitive ramie cultivars were chosen for investigating their genetic diversity by RAPD. 25 ramdom primers were used to amplify the total DNA from these genotypes. The results showed that 12 primers produced repoducible amplified products and the length of the products ranged 0.6～ 5.15 kb . Total 90 bands were identified, of which, 11 bands were common in all varieties and 79 bands were polymorphic. The polymorphic bands accounted for 87.78% of the total amplified bands. According to the polymorphism, 12 ramie cultivars could be divided into two types and three groups. This outcome was corresponding to their geographical distribution, but drought resistance genotypes were clusted in different type groups.
出处
《作物学报》
CAS
CSCD
北大核心
2002年第2期254-259,共6页
Acta Agronomica Sinica
基金
中国农业科学院院长基金资助
