摘要
目的 探讨大鼠局灶性脑缺血再灌注后受损伤的神经细胞和血管内皮细胞凋亡 ,以及Bcl 2和Bax蛋白表达与再灌注时间的关系。 方法 应用原位末端标记 (TUNEL)技术和免疫组织化学方法 ,分别观察脑缺血再灌注 2h、6h、12h、2 4h、2d、3d、7d、14d和 2 1d等不同时间点神经细胞和血管内皮细胞凋亡数及Bcl 2和Bax蛋白的表达。 结果 1.脑缺血周围区 ,再灌注 2h神经细胞和内皮细胞凋亡开始明显增多 ,12～ 2 4h达高峰 ,之后逐渐减少 ,7～ 14d降至假手术组水平 ;血管内皮细胞凋亡迟于神经元凋亡约 12h。 2 .Bcl 2蛋白表达于缺血再灌注 2h开始逐渐增强 ,12～ 2 4h达高峰 ,之后逐渐下降 ,至 7～ 14d接近假手术组水平 ;3.Bax蛋白表达于缺血再灌注 6h开始逐步增高 ,2 4～ 4 8h达高峰 ,之后逐渐下降 ,至 14d与假手术组已无显著性差异。 4 .Bcl 2表达与细胞凋亡的时相变化基本一致 ,Bax表达时相迟于细胞凋亡。 结论 细胞凋亡是脑缺血再灌注损伤细胞死亡的形式之一 ,血管内皮细胞凋亡迟于神经细胞凋亡 ,Bcl 2和Bax参与细胞凋亡的调节。
Objective To study the time relationships between apoptosis of neurons and endotheliocytes with the expression of Bcl 2 and Bax after reperfusion of focal cerebral ischemia in rats. Methods Coronal sections of brain were analyzed using an in situ terminal deoxynucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nick end labeling(TUNEL)and immunohistochemical staining methods to observe apoptosis of neurons and endotheliocytes,and expression of Bcl 2 and Bax after reperfusion(2,6,12,24hours and 2,3,7,14,21 days)of focal cerebral ischemia. Results 1.In the ischemic penumbra,apoptotic cells were increased at 2h after reperfusion,peaked at 12 24?h,then decreased successfully for 7 14?days.The time of apoptotic endothelial cells was 12?h later than that of apoptotic neurons.2.The expression of Bcl 2 protein began at 2?h after reperfusion,peaked at 12 24?h,and decreased for 7 14?days.3.Bax protein expressed from 6?h after reperfusion;peaked at 24 48?h,and lasted for 14days.4.The time phase of Bcl 2 expression was similar to the Bax is but later than it. Conclusion\ Apoptosis was a pattern of cell death after cerebral ischemia/reperfusion.The time of apoptotic endothelial cells was later than that of apoptotic neurons.Bcl 2 and Bax play a regulatory role in the apoptotic process.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第2期151-156,共6页
Acta Anatomica Sinica
基金
山东省自然科学基金资助项目 (Q97C0 112 5 )
