摘要
目的 探讨整合素α６在肝窦毛细血管化时的表达情况。方法 皮下注射四氯化碳制备大鼠肝纤维化模型，进行层粘连蛋白（ＬＮ）及其整合素受体α６免疫组织化学检测及整合素α６斑点免疫印迹研究。结果 动态观察了 ＬＮ在肝纤维化时沿肝窦在Ｄｉｓｓｅ间隙沉积形成肝窦毛细血管化；正常时整合素α６局限于汇管区血管内皮和胆管内皮细胞膜上，窦内皮细胞（ＳＥＣ）上无表达，肝窦毛细血管化时，ＳＥＣ出现整合素α６阳性表达沿肝窦连续分布，整合素α６在纤维化时组织中含量明显高于正常（Ｐ＜０．０５）。结论 肝窦毛细血管化时ＳＢＣ出现整合素α６亚基的诱导表达。
Objective To explore the expression of integrin α6 in hepatic sinusoidal capillaration. Methods The rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin α6 subunit was observed by immunohistochemistry and dot immuno-blotting. Results We observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin α6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin α6 was detected in a continuous pattern along the sinusoids, the content of integrin α6 was significantly higher in fibrotic liver tis- sues than in normal liver tissues as measured by dot immuo-blotting (P<0.05). Conclusions During fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin α 6 on SEC membranes.
出处
《中华肝脏病杂志》
CAS
CSCD
2002年第2期90-92,共3页
Chinese Journal of Hepatology
基金
国家自然科学基金(39700068)
