摘要
目的 :研究河南地区部分汉族人群中vWA和FESFPS基因座遗传多态性 ,获得vWA和FESFPS基因座的群体遗传学数据。方法 :应用聚合酶链反应 (PCR)结合聚丙烯酰胺凝胶电泳分型方法 ,分别观察河南地区 2 2 8例无血缘关系汉族人的vWA和FESFPS基因座的基因型分布。结果 :河南地区汉族群体中vWA基因座存在 8个等位基因 ,杂合度为 0 .76 6 6 ,个人识别能力为 0 .8913,非父排除率为 0 .5 4 3。FESFPS基因座存在 7个等位基因 ,杂合度为0 .7777,个人识别能力为 0 .70 0 8,非父排除率为 0 .4 6 0 3,2个位点基因型分布均符合Hardy Weinberg平衡。结论 :vWA和FESFPS位点均是高杂合度 ,高鉴别能力的遗传标记系统 ,具有较高的法医学应用价值。
Aim:To study the genetic polymorphism at vWA and FESFPS loci for partial Han people in Henan Province and to obtain population genetic data for vWA and FESFPS loci. Methods: Multiplex PCR technique and PAGE electrophoresis were used to detect the genotype frequencies of vWA and FESFPS loci for 228 cases of Han People without kinship.Results: Eight alleles of vWA and 7 alleles of FESFPS were identified. The expected heterozygosity of vWA and FESFPS were 0.766 6 and 0.777 7, respectively. The discrimination power (DP) was 0.891 3 for vWA and 0.700 8 for FESFPS. The power exclusions (PE) was 0.543 for vWA and 0.460 3 for FESFPS. The genotypes of both loci were accorded with Hardy Weinberg equilibrium. Conclusion: The results show that vWA and FESFPS loci are valuable genetic marker system in the areas of forensic medicine and genetics.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2002年第2期161-163,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省自然科学基金资助项目 0 1110 2 2 3 0 0
