摘要
探讨重组人骨形成蛋白 2 (rhBMP 2 )和地塞米松 (Dex)单独和联合作用对骨髓基质细胞 (BMSC)增殖和分化的影响。利用四唑盐比色法(MTT)、细胞总蛋白考马斯亮蓝测定法及碱性磷酸酶 (ALP)检测试剂盒分别测定不同浓度的rhBMP 2和Dex单独和联合作用后BMSC的增殖和分化情况。结果显示 ,rhBMP 2对BMSC的增殖和ALP的表达均有促进作用 ,Dex促进ALP的表达 ,但高浓度的Dex对BMSC有增殖抑制 ,rhBMP 2和Dex联合作用后BMSC的增殖和ALP活性较单独作用有更明显的升高。提示rhBMP
The present study was conducted to investigate the combined effects of recombinant human bone morphogenetic protein 2 (rhBMP 2) and dexamethasone (Dex) on the proliferation and differentiation of bone marrow stromal cells (BMSC). BMSCs were subjected to the treatment with different concentrations of rhBMP 2, Dex, or a mixture of rhBMP 2 and Dex, then proliferation and differentiation of the attached cells were measured with methylthiazol tetrazolium (MTT) method, Coomassie brilliant blue assay and alkaline phosphatase (ALP) measurement kit. The results showed that with treatment of rhBMP 2 alone could promote the proliferation of BMSCs and upregulate the expression of ALP in BMSCs. Treatment with Dex alone could also augment the expression of ALP, whereas high concentration of Dex could inhibit the proliferation of BMSC. Moreover, significantly greater differentiation of BMSCs were found in combined treatment group compared with individual treatment groups. The above results suggested that rhBMP 2 and Dex could synergistically promote the proliferation of BMSC and its differentiation to osteoblast.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第4期333-335,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家"973"项目资助课题 (编号G1 9990 5430 8)
